Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Kurthia massiliensis strain:JC30

ABSTRACT: Kurthia sp. JC30 Genome Sequencing

PROVIDER: PRJEA70831 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	CAEU01.dat.gz	Other
	CAEU01.fasta.gz	Fasta.gz
	CAEU01.master.dat	Other
	CAEU01.dat.gz	Other

Items per page:

1 - 4 of 4

Similar Datasets

Kurthia senegalensis strain:JC8E

Project description:Kurthia sp. JC8E genome sequencing

| PRJEA70835 | ENA

Kurthia sp. YJT4

Project description:Kurthia sp. YJT4 Genome sequencing

| PRJNA974937 | ENA

Kurthia sp. Dielmo

Project description:Kurthia sp. Dielmo Genome sequencing

| PRJEA70549 | ENA

Kurthia sp. 3B1D

Project description:Kurthia sp. 3B1D Genome sequencing and assembly

| PRJNA264126 | ENA

Kurthia sp. 11kri321

Project description:Kurthia sp 11kri321 genome sequencing and assembly

| PRJNA301103 | ENA

A programmable pAgo nuclease with universal guide and target specificity from the mesophilic bacterium Kurthia massiliensis

Project description:Argonaute proteins are programmable nucleases that are found in both eukaryotes and prokaryotes and provide defense against invading genetic elements. Although some prokaryotic Argonautes (pAgos) were shown to recognize RNA targets in vitro, the majority of studied pAgos have strict specificity toward DNA, which limits their practical use in RNA-centric applications. Here, we describe a unique pAgo nuclease, KmAgo, from the mesophilic bacterium Kurthia massiliensis that can be programmed with either DNA or RNA guides and can precisely cleave both DNA and RNA targets. KmAgo binds 16-20 nt long 5’-phosphorylated guide molecules with no strict specificity for their sequence and is active in a wide range of temperatures. In bacterial cells, KmAgo is loaded with small DNAs with no obvious sequence preferences suggesting that it can uniformly target genomic sequences. Mismatches between the guide and target sequences greatly affect the efficiency and precision of target cleavage, depending on the mismatch position and the nature of the reacting nucleic acid. Target RNA cleavage by KmAgo depends on the formation of secondary structure indicating that KmAgo can be used for structural probing of RNA. These properties of KmAgo open the way for its use for highly specific nucleic acid detection and cleavage.

2021-03-02 | GSE168010 | GEO

Kurthia gibsonii strain:ND2

Project description:Kurthia gibsonii strain:ND2 Genome sequencing

| PRJNA1086691 | ENA

Kurthia zopfii strain:DSM 20580

Project description:Kurthia zopfii DSM 20580 genome sequencing

| PRJNA463388 | ENA

Kurthia gibsonii strain:B83

Project description:Kurthia gibsonii strain:B83 Genome sequencing and assembly

| PRJNA492835 | ENA

Kurthia gibsonii

Project description:Kurthia gibsonii overview