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Gene expression profiles of null Pacman/Xrn1 mutant Drosophila L3 wing imaginal discs

ABSTRACT: Gene expression profiles of null Pacman/Xrn1 mutant Drosophila L3 wing imaginal discs

PROVIDER: PRJEB10035 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR973488_1.fastq.gz	Fastqsanger.gz
	ERR973488_2.fastq.gz	Fastqsanger.gz
	ERR973489_1.fastq.gz	Fastqsanger.gz
	ERR973489_2.fastq.gz	Fastqsanger.gz
	ERR973490_1.fastq.gz	Fastqsanger.gz

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Gene expression profiles of null Pacman/Xrn1 mutant Drosophila L3 wing imaginal discs

Project description:Two pcm null alleles (pcm14 and pcm15) were sequenced alongside respective wild-type controls (wild-type 1 and wild-type 2). Each genotype was replicated 3 times. Read depth was 12-17 million reads per replicate. Total RNA was prepared from Drosophila L3 larval wing imaginal discs.

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Project description:Genotype: FRT82B Method: Staged wild type wing imaginal discs were dissected and transferred to DPBS. The wing imaginal discs were dissociated in 0.25% Trypsin-EDTA solution at 37 ℃ for 10 min. Cells were then washed in DPBS and passed through 35μm filter before library preparation. Construction of 10x single cell libraries and sequencing on Illumina Hiseq platform were performed by Novogene.

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