Project description:RNA was isolated from 14 day old Arabidopsis Col-0 seedlings in three biological repeats using the RNeasy Plant Mini Kit (Qiagen) and DNase I treated (Promega). A Trueseq RNA-Seq library (Illumina) was compiled and sequenced as 50-bp single read using Illumina HiSeq 2000.
Project description:Wild type Columbia and serrate-1 globular stage embryos were sequenced in order to profile miRNAs which are expressed in embryogenesis in Arabidopsis thaliana
Project description:Identification of nutrient-specific microRNA expressed in Arabidopsis seedlings Small RNA sequencing of Arabidopsis wild type ecotype Columbia (Col-0) seedling under different nutrient limitation conditions
Project description:Comparison of TopHat alignments and assessment of spurious splice junctions for 32nt and 76nt read lengths. Total RNA from 2-week-old Arabidopsis thaliana (ecotype Columbia) seedlings grown on MS plates was isolated using RNeasy Plant Mini Kit from Qiagen. To remove any contaminating DNA, RNA was treated with DNAse. Isolation of poly (A) mRNA and preparation of cDNA library were carried out using the Illumina TrueSeq RNA kit. Sequencing (72 cycle) was done on Illumina Genome Analyzer II.
Project description:To investigate the differential gene expression between wild type and rli2 RNAi, we collected samples of the seedlings from WT and rli2 RNAi plants with three biological replicates to perform RNA-seq assay.
Project description:Transcriptional profiling of 6-day-old seedlings of Arabidopsis wild type control and zrf1 mutants is performed using Agilent's Whole Arabidopsis Gene Expression Microarray (4x44K).
Project description:This experiment has been annotated by TAIR (http://arabidopsis.org). In this experiment, different tissue preparations of wild type Columbia-0 Arabidopsis thaliana plants were hybridized and run on the ATH1 Affymetrix platform. Experimenter name = Chris Somerville Experimenter phone = 650-325-1521 ext203 Experimenter fax = 650-325-6857 Experimenter address = Plant Biology Experimenter address = Carnegie Institution Experimenter address = 260 Panama Street Experimenter address = Stanford Experimenter zip/postal_code = CA 94305-1297 Experimenter country = USA Keywords: organism_part_comparison_design;