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Heligmosomoides bakeri Genome sequencing

ABSTRACT: Genomic assembly of nematode Heligmosomoides bakeri (formerly known as Heligmosomoides polygyrus bakeri), as part of the 50 Helminth Genomes Initiative sequencing of the parasitic worms that have the greatest impact on human, agricultural and veterinary disease and cause significant global health issues particularly in the developing world, or those used as model organisms.

PROVIDER: PRJEB1203 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	UZAH01.dat.gz	Other
	UZAH01.fasta.gz	Fasta.gz
	UZAH01.master.dat	Other
	UZAH01.dat.gz	Other

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Genome sequencing of Heligmosomoides polygyrus bakeri

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The production of Excretory-Secretory molecules from Heligmosomoides polygyrus bakeri fourth stage larvae varies between mixed and single sex cultures.

Project description:In this study, the composition of ES of male and female L4 stage Heligmosomoides polygyrus bakeri in the presence (cultured together) or absence (cultured alone) of the opposite sex was examined using mass spectrometry.

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Project description:We report the small RNA content of extracellular vesicles (EVs) from adult parasite Trichuris muris and Heligmosomoides bakeri

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Transcriptome analysis of Intestinal epithelial cells after Heligmosomoides polygyrus bakeri (Hpb) infection

Project description:Transcriptome analysis of Intestinal epithelial cells after Heligmosomoides polygyrus bakeri (Hpb) infection

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Excretory/secretory microRNAs from the gastrointestinal nematode Heligmosomoides polygyrus bakeri in culture.

Project description:Excretory/secretory microRNAs from the gastrointestinal nematode Heligmosomoides polygyrus bakeri in culture.

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Mus musculus intestinal epithelial MODE-K cells incubated with Heligmosomoides bakeri secreted extracellular vesicles (EVs)

Project description:Small RNAs are emerging as important molecules for cross-species communication. Thanks to available and affordable sequencing technologies it is now possible to sequence small RNAs (sRNA-Seq) present in samples of interacting organisms. A first step when analyzing sRNA-Seq of two interacting species is to determine which sequences are being produced by which organism. Due to their small size (18-30), small RNAs could easily map to both host and parasite genomes. Here we produced data for Mus musculus intestinal epithelial cells treated with Extracellular Vesicles (EV) produced by the parasitic nematode Heligmosomoides bakeri.

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microarray analysis of bone marrow derived macrophages stimulated with helminth larvae and immune serum

Project description:Mouse bone marrow derived macrophages were stimulated with L3 larvae of the helminth Heligmosomoides polygyrus (Hp) (500 L3 /1 mio cells) in the presence or absence of immune serum (1:50 v:v) from challenge-Hp-infected mice.

2013-12-31 | E-MEXP-3920 | biostudies-arrayexpress

Duodenal tissues and mesenteric lymph nodes from wild-type (BALB/c) and MIF deficient mice were compared in the steady state and at day 5 following infection with the gastrointestinal helminth parasite Heligmosomoides polygyrus

Project description:We have found that macrophage migration inhibitory factor (MIF) is essential for the development of effective immunity to the intestinal helminth Heligmosomoides polygyrus, even following vaccination which induces sterile immunity in wild-type mice. In the context of a Type 2 infection, MIF plays a critical role in polarizing macrophages into the protective alternatively-activated phenotype, and that STAT3 signaling may make a previously unrecognized contribution to immunity to helminths.

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