Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Complete genome sequence of Enterococcus faecium ATCC 700221

ABSTRACT: Complete genome sequence of Enterococcus faecium ATCC 700221 using PacBio corrrected by Illumina short reads.

PROVIDER: PRJEB12743 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome mapping of Enterococcus faecalis and Enterococcus faecium

Project description:The enterococci comprise a genus of 49 low-GC content Gram-positive commensal species within the Firmicutes phylum that are known to occupy diverse habitats, notably the gastrointestinal core microbiota of nearly every phylum, including human. Of particular clinical relevance are two rogue species of enterococci, Enterococcus faecalis and the distantly related Enterococcus faecium, standing among the nefarious multi-drug resistant and hospital-acquired pathogens. Despite increasing evidence for RNA-based regulation in the enterococci, including regulation of virulence factors, their transcriptome structure and arsenal of regulatory small sRNAs (sRNAs) are not thoroughly understood. Using dRNA-seq, we have mapped at single-nucleotide resolution the primary transcriptomes of E. faecalis V583 and E. faecium AUS0004. We identified 2517 and 2771 transcription start sites (TSS) in E. faecalis and E. faecium, respectively. Based on the identified TSS, we created a global map of s70 promoter motifs. We also revealed features of 5’ and 3’UTRs across the genomes. The transcriptome maps also predicted 150 and 128 sRNA candidates in E. faecalis and E. faecium, respectively, some of which have been identified in previous studies and many of which are new. Finally, we validated several of the predicted sRNAs by Northern Blot in biologically relevant conditions. Comprehensive TSS mapping of two representative strains will provide a valuable resource for the continued development of RNA biology in the Enterococci.

2020-11-25 | GSE115009 | GEO

Grad-seq Enterococcus faecalis and faecium

Project description:Grad-seq in Enterococcus faecalis V583 and Enterococcus faecium AUS004.

2022-11-28 | PXD031577 | Pride

Proteomic analysis Enterococcus faecium

Project description:Proteomic analysis Enterococcus faecium samples to assess proteomic alterations (20220922_NSco_Stinear)

2024-08-15 | PXD039831 | Pride

Bacteriocin producing Enterococcus faecium

Project description:Proteomic Characterization of Bacteriocin-Producing Enterococcus faecium Strains from foodstuffs

2023-03-11 | PXD037241 | Pride

Enterococcus faecium EnGen0376

Project description:Enterococcus faecium Pat2-2 Genome PacBio/Illumina sequencing

| PRJNA191894 | ENA

Enterococcus faecium EnGen0375

Project description:Enterococcus faecium Pat2-1 Genome PacBio/Illumina sequencing

| PRJNA191893 | ENA

Enterococcus faecium EnGen0377

Project description:Enterococcus faecium Pat2-3 Genome PacBio/Illumina sequencing

| PRJNA191895 | ENA

Enterococcus faecium E1162 transcriptome 25 C vs 37 C

Project description:The transcriptome of Enterococcus faecium E1162 growing in Brain heart Infusion Broth was compared in the mid-exponential growth phase (A660 = 0.3) at 25 C and 37 C.

2013-09-01 | E-MEXP-3941 | biostudies-arrayexpress

Subinhibitory concentrations of ciprofloxacin enhance antimicrobial resistance and pathogenicity of Enterococcus faecium

Project description:Enterococcus faecium has emerged as a major opportunistic pathogen for two decades, with the spread of hospital-adapted multidrug-resistant clones. Members of the intestinal microbiota, they are subjected to numerous bacterial stresses, including antibiotics at subinhibitory concentrations (SICs). Since fluoroquinolones are extensively prescribed, SICs are very likely to occur in vivo with potential effects on bacterial metabolism with subsequent modulation of opportunistic traits. The aim of the study was to evaluate globally the impact of subinhibitory concentrations (SICs) of ciprofloxacin on antimicrobial resistance and pathogenicity of E. faecium. Transcriptomic analysis was performed by RNA-seq (HiSeq 2500, Illumina) using the vanB-positive reference strain E. faecium Aus0004 in the absence or presence of ciprofloxacin SIC (0.38 mg/L, i.e. MIC 1/8). Several genetic and phenotypic tests were used for validation. In the presence of ciprofloxacin SIC, 196 genes were significantly induced whereas 286 were significantly repressed, meaning that 16.8% of the E. faecium genome was altered. Amongst upregulated genes, EFAU004_02294 (fold change of 14.3) encoded a protein (EfmQnr) homologue of Qnr proteins involved in quinolone resistance in Gram-negative bacilli. Its implication in intrinsic and adaptive FQ resistance in E. faecium was experimentally ascertained. Moreover, EFAU004_02292 coding for the collagen adhesin Acm was also induced by SIC of ciprofloxacin (fold change of 8.2), and higher adhesion capabilities were demonstrated phenotypically. Both Efmqnr and Acm determinants may play an important role in the transition from a commensal to a pathogenic state of E. faecium that resides in the gut of patients receiving a fluoroquinolone therapy.

2017-02-04 | GSE94507 | GEO

Enterococcus faecium ATCC 8459 = NRRL B-2354

Project description:Enterococcus faecium ATCC_8459 PacBio/Illumina Sequencing Project

| PRJNA191899 | ENA