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Two centuries of cyanobacterial diversity recovered from sediments

ABSTRACT: Sedimentary DNA reveals cyanobacterial community diversity over 200 years in two perialpine lakes

PROVIDER: PRJEB13044 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR1314330_1.fastq.gz	Fastqsanger.gz
	ERR1314330_2.fastq.gz	Fastqsanger.gz
	ERR1314340_1.fastq.gz	Fastqsanger.gz
	ERR1314340_2.fastq.gz	Fastqsanger.gz
	ERR1314350_1.fastq.gz	Fastqsanger.gz

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Project description:Previous molecular and mechanistic studies have identified several principles of prokaryotic transcription, but less is known about the global transcriptional architecture of bacterial genomes. Here we perform a comprehensive study of a cyanobacterial transcriptome, that of Synechococcus elongatus PCC 7942, generated by combining three high-resolution data sets: RNA sequencing, tiling expression microarrays, and RNA polymerase chromatin immunoprecipitation (ChIP) sequencing. We report absolute transcript levels, operon identification, and high-resolution mapping of 5' and 3' ends of transcripts. We identify several interesting features at promoters, within transcripts and in terminators relating to transcription initiation, elongation, and termination. Furthermore, we identify many putative non-coding transcripts. We provide a global analysis of a cyanobacterial transcriptome. Our results uncover insights that reinforce and extend the current views of bacterial transcription. RNA Sequencing of the cyanobacterium Synechococcus elongatus PCC 7942 RNA polymerase ChIP Sequencing of the cyanobacterium Synechococcus elongatus PCC 7942 Tiling Microarray of the cyanobacterium Synechococcus elongatus PCC 7942

2011-05-16 | E-GEOD-29264 | biostudies-arrayexpress

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