Project description:Transcriptional profiling of Rhodopseudomonas palustris (R. palustris) comparing cbbT1 over-expressing strain with cbbT2 over-expressing strain. Goal was to discriminate the molecular mechanisms between transketolase I (cbbT1) and transketolase II (cbbT2). R.palustris is a purple non-sulfur anoxygenic phototrophic bacterium and transketolase (cbbT1 and cbbT2) is a key enzyme involved in the CBB cycle. Here, we investogated the functions of transketolase isoforms I (cbbT1) and II (cbbT2) in R. palustris through transcriptional profiling and other functional assays.
Project description:In this study, we provide a time-series analysis of the transcriptional response of Gemmatimonas phototrophica AP64T during the dark-to-light transition under aerobic and semiaerobic conditions. By analysing its transcriptome, focussing especially on PS-related genes, we tested the hypothesis that G. phototrophica might constitute an example of an anoxygenic phototroph on its evolutionary pathway from anaerobic to aerobic life-style.
Project description:In this study, we provide a time-series analysis of the transcriptional response of Gemmatimonas phototrophica AP64T during the dark-to-light transition under aerobic and semiaerobic conditions. By analysing its transcriptome, focussing especially on PS-related genes, we tested the hypothesis that G. phototrophica might constitute an example of an anoxygenic phototroph on its evolutionary pathway from anaerobic to aerobic life-style.
Project description:In this study, we provide a time-series analysis of the transcriptional response of Gemmatimonas phototrophica AP64T during the dark-to-light transition under aerobic and semiaerobic conditions. By analysing its transcriptome, focussing especially on PS-related genes, we tested the hypothesis that G. phototrophica might constitute an example of an anoxygenic phototroph on its evolutionary pathway from anaerobic to aerobic life-style.
Project description:Transcriptional profiling of Rhodopseudomonas palustris (R. palustris) comparing cbbT1 over-expressing strain with cbbT2 over-expressing strain. Goal was to discriminate the molecular mechanisms between transketolase I (cbbT1) and transketolase II (cbbT2). R.palustris is a purple non-sulfur anoxygenic phototrophic bacterium and transketolase (cbbT1 and cbbT2) is a key enzyme involved in the CBB cycle. Here, we investogated the functions of transketolase isoforms I (cbbT1) and II (cbbT2) in R. palustris through transcriptional profiling and other functional assays. Four-condition experiment, cbbT1 over-expressing cells, cbbT2 over-expressing cells, Negative control strain with empty plasmid, Wild type strain.
