Project description:The objective of this project is to investigate the gene expression pattern relevant for the utilization of different carbon sources in the yeast Candida intermedia CBS141442, as well as assess the effect of the presence of oxygen.
Project description:This study compares gene expression in three strains (CPL2H1, CPL2H1 gtf1/gtf1 and CPL2H1 otf1/otf1) with the genetic background of Candida parapsilosis CLIB 214 (CBS 604). Total RNA was isolated using hot acid phenol extraction. A TruSeq stranded mRNA library was sequenced on an Illumina NovaSeq 6000 system.
