Project description:We used Candida albicans lab strain SC5314 to obtain tunicamycin adaptors. We did whole genome sequencing of the adaptors and the parent as well.
Project description:We treated Candida albicans lab strain SC5314 with fluorouracil and we did whole genome sequencing of 24 adaptors as well as the parent
Project description:Miltefosine has been identified for its antifungal properties, yet the mechanisms underlying its action and the development of resistance remain unclear. In this study, we induced drug resistance in various Candida species through concentration gradient exposure, successfully obtaining a miltefosine-resistant strain of Candida glabrata. Whole-genome sequencing revealed a premature stop codon mutation in the OSH2 gene within the resistant strain. To further investigate the function of the Osh2, we deleted it in Candida albicans, observing a significant increase in miltefosine resistance in the knockout strain. RNA-seq and lipidomics analyses indicated that Osh2 influences the ergosterol biosynthetic pathway by regulating zymosterol transport, thereby mediating miltefosine resistance. Additionally, exogenous supplementation of zymosterol significantly enhanced the resistance of Candida to miltefosine. Further studies revealed that deletion of the ERG11 gene, a rate-limiting enzyme in the zymosterol synthesis pathway, rendered the strain highly sensitive to miltefosine, while deletion of the ERG6 gene, which catalyzes the conversion of zymosterol to fecosterol, led to miltefosine resistance. This study is the first to elucidate that the Osh2 gene mediates miltefosine resistance in Candida by regulating zymosterol transport and affecting the ergosterol biosynthetic pathway, providing new theoretical insights into the mechanism of action of miltefosine and strategies to overcome fungal resistance.
Project description:In Candida albicans, one strain (TJ1031) has segmental monosomy of chromosome R from rDNA to the right tolemere. This strain was unstable, yielding small and large colonies on YPD plate. Randomly 5 large colonies were sequenced.
Project description:Several aneuploid Candida glabrata strains, each bearing a unique karyotype, were spread on YPD plates. They all exhibited colony size vairations. Randmly 10 large colonies from each strain were sequenced.