Project description:Reduction in visceral adipose tissue (VAT) mass reduces body weight and metabolic disease risk in obese patients. However surgical removal of VAT is highly invasive and thus not clinically feasible. We developed an injectable ice slurry for selective reduction of adipose tissue through cryolipolysis. The aim of this study was to investigate safety, feasibility and mechanism of ice slurry-induced cryolipolysis of VAT. Perigonadal VAT in diet-induced obese mice and rats was subjected to slurry or sham treatment. Body weight and blood chemistry were monitored for 56 days post-treatment. Histological analysis and molecular studies were performed to elucidate mechanisms of fat reduction. Treatment of VAT was well tolerated in all animals. Slurry induced adipocyte cell death via selective cryolipolysis; significant weight loss was noted at day 21 post-treatment. RNA sequencing from treated VAT samples showed increased expression of genes involved in inflammation, immune response, collagen biosynthesis and wound healing, and decreased expression of adipokines. This study demonstrates that slurry treatment is safe and effective in inducing cryolipolysis of VAT and subsequent weight loss in rodents. Ice slurry is promising as a minimally-invasive treatment to reduce visceral adipose tissue.

2023-09-12 | GSE240955 | GEO

Whole genome sequencing of Burkholderia contaminans sequential isolates

Project description:The data represent whole genome sequencing of two sequential isolates of B. contaminans ST872 that have been retrieved form a cystic fibrosis patient during different phases of chronic pulmonary infection.

2016-05-13 | E-MTAB-4649 | biostudies-arrayexpress

YerA41, a Yersinia ruckeri bacteriophage: determination of non-sequencable bacteriophage genome and investigation of bacterial response to infection.

Project description:YerA41 is a myoviridae bacteriophage that was originally isolated due its ability to infect Yersinia ruckeri bacteria, the causative agent of enteric redmouth disease of salmonid fish. Several attempts to determine its genomic DNA sequence using traditional and next generation sequencing technologies failed, indicating that the phage genome is modified such way that it is an unsuitable template for PCR amplification and sequencing. To determine the YerA41 genome sequence we isolated RNA from phage-infected Y. ruckeri cells at different time points post-infection, and sequenced it. The host-genome specific reads were substracted and de novo assembly was performed on the unaligned reads.

2020-03-04 | GSE146319 | GEO

Caulobacter bacteriophage

Project description:environmental bacteriophage isolates that infect Caulobacter hosts genome sequencing

| PRJNA299660 | ENA

Bacteriophage resistant Enterococcus faecalis isolates

Project description:Bacteriophage resistant Enterococcus faecalis isolates with mutations in cell wall polysaccharide synthesis genes

| PRJEB30526 | ENA

Bacteriophage sp.

Project description:Bacteriophage sp. Genome sequencing

| PRJNA254443 | ENA

Bacteriophage sp.

Project description:Bacteriophage sp. Genome sequencing