Project description:Whole genome microarray were used to generate the transcriptional profile of C. parapsilosis UPC2 delete in hypoxia. RNA was isolated from CLIB214 or upc2 delete grown in YPD medium in hypoxia at 30 degree Celsius for 2 h, and labeled with Cy3 or Cy5. Four independent biological replicates were compared. 2 dye swaps were perfomed so that 2 out of 4 samples isolated from CLIB214 in hypoxic culture were labeled with Cy3, and 2 were labeled with Cy5
Project description:Whole genome microarray were used to generate the transcriptional profile of C.albicans UPC2 delete in hypoxia. RNA was isolated from DAY286 or UPC2 delete grown in YPD medium in hypoxia at 30 degree Celsius for 3.5 h, and labeled with Cy3 or Cy5. Nine independent biological replicates were compared. 3 dye swaps were perfomed so that 6 out of 9 samples isolated from DAY286 in hypoxic culture were labeled with Cy3, and 3 were labeled with Cy5.