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Mcr-1 and mcr-2 variant genes identified in Moraxella species isolated from pigs.

ABSTRACT: mcr-1 and mcr-2 variant genes identified in Moraxella species isolated from pigs.

PROVIDER: PRJEB15347 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1797991_1.fastq.gz	Fastqsanger.gz
	ERR1797991_2.fastq.gz	Fastqsanger.gz
	ERR1797992_1.fastq.gz	Fastqsanger.gz
	ERR1797992_2.fastq.gz	Fastqsanger.gz
	ERR1797993_1.fastq.gz	Fastqsanger.gz

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mcr-1 and mcr-2 variant genes identified in Moraxella species isolated from pigs in Great Britain from 2014 to 2015.

Project description:ObjectivesTo determine the occurrence of mcr-1 and mcr-2 genes in Gram-negative bacteria isolated from healthy pigs in Great Britain.MethodsGram-negative bacteria (n = 657) isolated from pigs between 2014 and 2015 were examined by WGS.ResultsVariants of mcr-1 and mcr-2 were identified in Moraxella spp. isolated from pooled caecal contents of healthy pigs at slaughter collected from six farms in Great Britain. Other bacteria, including Escherichia coli from the same farms, were not detected harbouring mcr-1 or mcr-2. A Moraxella porci-like isolate, MSG13-C03, harboured MCR-1.10 with 98.7% identity to MCR-1, and a Moraxella pluranimalium-like isolate, MSG47-C17, harboured an MCR-2.2 variant with 87.9% identity to MCR-2, from E. coli; the isolates had colistin MICs of 1-2 mg/L. No intact insertion elements were identified in either MSG13-C03 or MSG47-C17, although MSG13-C03 harboured the conserved nucleotides abutting the ISApl1 composite transposon found in E. coli plasmids and the intervening ∼2.6 kb fragment showed 97% identity. Six Moraxella osloensis isolates were positive for phosphoethanolamine transferase (EptA). They shared 62%-64.5% identity to MCR-1 and MCR-2, with colistin MICs from 2 to 4 mg/L. Phylogenetic analysis indicated that MCR and EptA have evolved from a common ancestor. In addition to mcr, the β-lactamase gene, blaBRO-1, was found in both isolates, whilst the tetracycline resistance gene, tetL, was found in MSG47-C17.ConclusionsOur results add further evidence for the mobilization of the mcr-pap2 unit from Moraxella via composite transposons leading to its global dissemination. The presence of mcr-pap2 from recent Moraxella isolates indicates they may comprise a reservoir for mcr.

| S-EPMC5890717 | biostudies-literature

Co-occurrence of mcr-1 and mcr-3 in E.coli isolated from humans and pigs in Thailand

Project description:Co-occurrence of mcr-1 and mcr-3 in E.coli isolated from healthy humans and pigs in Thailand

| PRJEB39885 | ENA

mcr-1-encoding E. coli isolates from German pigs

Project description:mcr-1-encoding E. coli isolates from German pigs

| PRJEB21879 | ENA

Gene expression microarray data by Moutan Cortex Radicis (MCR) in the LPS-stimulated human gingival fibroblast

2012-06-28 | GSE38977 | GEO

Gene Expression Profile of Moraxella catarrhalis Grown in Pooled Human Sputum

2014-02-02 | GSE27059 | GEO

Analysis of the Zur Regulon of Moraxella catarrhalis

Project description:This series of microarrays explores the genes regulated by the Zur gene of Moraxella catarrhalis.

2014-03-23 | GSE28151 | GEO

Gene Expression Profile of Moraxella catarrhalis Grown in Pooled Human Sputum

Project description:Comparison of the gene expression profile of Moraxella catarrhalis grown in the presence of 20% pooled human sputum in chemically-defined medium relative to Moraxella catarrhalis grown in chemically-defined medium alone. Moraxella catarrhalis ATCC43617 was grown to mid-logarithmic phase either in the presence of 20% pooled human sputum in chemically-defined medium or in chemically-defined medium alone. Total RNA was extracted from bacterial cells exposed to each of these conditions and cDNA was generated for CyDye labelling. 3 biologic replicates were generated and each replicate underwent a dye swap (total of 6 experimental data collections). The gene expression profile reported is that of Moraxella catarrhalis grown in the presence of pooled human sputum in a chemically-defined medium relative to Moraxella catarrhalis grown only in the presence of the chemically-defined medium.

2014-02-02 | E-GEOD-27059 | biostudies-arrayexpress

Moraxella

Project description:Moraxella comparative genomics

| PRJNA477706 | ENA

Moraxella

Project description:Moraxella sp. overview

| PRJNA175328 | ENA

Gene expression microarray data by Moutan Cortex Radicis (MCR) in the LPS-stimulated human gingival fibroblast

Project description:The Moutan Cortex Radicis (MCR) has been used as an analgesic, sedative and anti-inflammatory agent. This study investigated the changes in gene expression by MCR treatment when stimulated with lipopolysaccharide (LPS) in cultured human gingival fibroblasts (HGFs) and the gene expression changes by the MCR when challenged with LPS using a microarray chip. Human gingival fibroblast were divided into three experimental groups; 1, C: Control, 2, LPS: LPS-treatment only, 3, MCR40: LPS- and MCR40-treatments. Total RNA was isolated from each experimental fibroblast (3 experimental group M-CM-^W 1 sample of each experimental group = total 3 samples).

2012-06-27 | E-GEOD-38977 | biostudies-arrayexpress

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