Project description:This is a comparative experiments of three barley genotypes harbouring allelic differences at a locus designated QRMC-3HS putatively implicated in the assembly of the microbial communities thriving at the root-soil interface, the so called rhizosphere microbiota. The RNA-seq experiment aimed at identify genes differentially regulated among the genotypes at the locus of interest. As the selected genotypes host contrasting microbiotas, we hypothesised that differentially expressed genes at the locus represent primary candidates for the trait of interest (i.e., microbiota recruitment).
Project description:Structure and functions of the bacterial root microbiota in wild and domesticated barley and signatures of positive selection in the rhizosphere metagenome
Project description:We investigated proteome and the involvement of barley MPK3 in response to flagellin peptide flg22, a well-known bacterial elicitor. We show that MPK3 knock out lines of barley (Hv MPK3 KO) exhibit constitutive downregulation of defence related proteins such as PR proteins belonging to thaumatin family and chitinases. Further proteomic analyses showed, that the same protein families were less prone to flagellin elicitation in MPK3 KO plants compared to wild types. These results positively correlated with chitinase activity analyses. Differential proteome also indicated possible root growth defects in the mutant plants, which was corroborated with early root growth retardation and altered short- and mid-term reactions to flg22 in terms of radial root expansion and root hair emergence.
Project description:In this study, we used transcriptomic and hormonomic approaches to examine drought-induced changes in barley roots and leaves and its rhizosphere. By studying hormonal responses, alternative splicing events in barley, and changes in the rhizosphere microbiome, we aimed to provide a comprehensive view of barley drought-adaptive mechanisms and potential plant-microbe interactions under drought stress. This approach improved our understanding of barley adaptive strategies and highlighted the importance of considering plant-microbe interactions in the context of climate change.
Project description:Waterlogging is a major abiotic stress causing oxygen depletion and carbon dioxide accumulation in the rhizosphere. Barley is more susceptible to waterlogging stress than other cereals. To gain a better understanding of the effect of waterlogging stress in barley, we carried out a genome-wide gene expression analysis in roots of Yerong and Deder2 barley genotypes under waterlogging and control (well-watered) conditions by RNA-Sequencing, using Illumina HiSeq™ 4000 platform.