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Transcriptomes and differential gene expression at the Arabidopsis shoot meristem during flowering

ABSTRACT: Transcriptomes and differential gene expression at the Arabidopsis shoot meristem during flowering

PROVIDER: PRJEB16325 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1698200_1.fastq.gz	Fastqsanger.gz
	ERR1698200_2.fastq.gz	Fastqsanger.gz
	ERR1698201_1.fastq.gz	Fastqsanger.gz
	ERR1698201_2.fastq.gz	Fastqsanger.gz
	ERR1698202_1.fastq.gz	Fastqsanger.gz

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Transcriptomes and differential gene expression at the Arabidopsis shoot meristem during flowering

Project description:We investigated the transcriptomes and differential gene expression at the Arabidopsis shoot meristem during flowering using INTACT reporter lines. Samples were collected in four biological replications.

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Project description:gnp07_regeneome_transdifferenciation - microdissection - Study of the moleculars mecanism during transdifferenciation of Root ApicalMeristem to Shoot Apical Meristem - middle of growth permits to induce transdifferenciation of root apical meristem to shoot apical meristem

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Transcriptomes and differential gene expression in the Arabidopsis shoot phloem companion cells during flowering

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Project description:DNA methylation is an epigenetic modification that specifies the basic state of pluripotent stem cells and regulates the developmental transition from stem cells to various cell types. In flowering plants, the shoot apical meristem (SAM) contains a pluripotent stem cell population which generates the aerial part of plants including the germ cells. Under appropriate conditions, the SAM undergoes a developmental transition from a leaf-forming vegetative SAM to an inflorescence- and flower-forming reproductive SAM. While SAM characteristics are largely altered in this transition, the complete picture of DNA methylation remains elusive. Here, by analyzing whole-genome DNA methylation of isolated rice SAMs in the vegetative and reproductive stages, we found that methylation at CHH sites is kept high, particularly at transposable elements (TEs), in the vegetative SAM relative to the differentiated leaf, and increases in the reproductive SAM via the RNA-dependent DNA methylation pathway. We also found that half of the TEs that were highly methylated in gametes had already undergone CHH hypermethylation in the SAM. Our results indicate that changes in DNA methylation begin in the SAM long before germ cell differentiation to protect the genome from harmful TEs.

2020-08-14 | PXD020608 | Pride

Transdifferentiation from root to shoot apical meristem

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Analysis of the Arabidopsis shoot meristem transcriptome during floral transition identifies distinct regulatory patterns and an LRR protein that promotes flowering

Project description:We sequenced cDNA synthesized from mRNA from 3 time points of a time course of Arabidopsis thaliana shoot apical meristems. Plants were grown two weeks in short days (time point +0LD) and then exposed to long days for 1 day (time point +1LD) and 3 days (time point +3LD). Meristem cells were collected using laser capture microdissection, to generate a meristem-specific time course of gene expression in the early stages of floral induction. The experiment was done in three biological replicates (called A, B, and C). Some repetitions of sequencing on a few samples (technical replicates) were also done Examination of mRNA levels in shoot apical meristems at 3 time points from the stage of vegetative meristem to the stage of a transition meristem, before the formation of floral meristems.

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