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DNA-seq of Candida albians strains engineered to mis-incorporate increasing levels of leucine at CUG codon, SYBARIS project

ABSTRACT: DNA-seq of Candida albians strains engineered to mis-incorporate increasing levels of leucine at CUG codon, SYBARIS project

PROVIDER: PRJEB1755 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR257695_1.fastq.gz	Fastqsanger.gz
	ERR257695_2.fastq.gz	Fastqsanger.gz
	ERR257696_1.fastq.gz	Fastqsanger.gz
	ERR257696_2.fastq.gz	Fastqsanger.gz
	ERR257697_1.fastq.gz	Fastqsanger.gz

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DNA-seq of Candida albians strains engineered with increasing levels of leucine mis-incorporation at CUG codon, SYBARIS project

Project description:The aim of this experiment is to test how atypical codon assignment (in our case Ser and Leu at CUG sites) flexibility can provide an effective mechanism to alter the genetic code. We have reengineered C. albicans strains to mis-incorporate increasing levels of Leu at protein CUG sites.

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Project description:While the “universal” genetic code is now known not to be universal, and stop codons can have multiple meanings, one regularity remains, namely that for a given sense codon there is a unique translation. Examining CUG usage in yeasts that have transferred CUG away from leucine, we here report the first example of dual coding: Ascoidea asiatica stochastically encodes CUG as both serine and leucine. This is deleterious as evidenced by CUG codons being rare, never at conserved serine/leucine residues and predominantly in lowly expressed genes. Related yeasts solve the problem by loss-of-function of one of the two tRNAs. This dual-coding is consistent with the tRNA-loss driven codon reassignment hypothesis and provides a unique example of a proteome that cannot be deterministically predicted.

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Project description:The standard genetic code is almost universal, and the evolutionary factors that caused a few organisms to deviate from it are poorly understood. We report that three independent changes of the genetic code occurred during the evolution of budding yeasts, each of which was a reassignment of the codon CUG from leucine to another amino acid. We identify five major yeast clades that differ by translating CUG as either Ser (2 clades), Ala (1 clade), or Leu (2 clades). The newly discovered Ser2 clade is in the final stages of transition from one genetic code to another. It appears to use only a novel tRNASer (tSCAG) to translate CUG codons, but the gene for the ancestral tRNALeu (tLCAG) is still intact in most species in the clade, consistent with the ‘ambiguous intermediate’ theory. We propose that the three parallel changes of the genetic code in yeasts were not driven by natural selection in favor of their effects on the proteome, but by selection to eliminate the ancestral tLCAG, possibly in response to a killer toxin.

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Chemotherapeutic agents and leucine deprivation induce codon-biased aberrant protein production in cancer

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