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Finding cellular origin of mouse kidney organoids

ABSTRACT: Finding cellular origin of mouse kidney organoids

PROVIDER: PRJEB17924 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1730310.fastq.gz	Fastqsanger.gz
	ERR1730311.fastq.gz	Fastqsanger.gz
	ERR1730312.fastq.gz	Fastqsanger.gz
	ERR1730313.fastq.gz	Fastqsanger.gz
	ERR1730314.fastq.gz	Fastqsanger.gz

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Project description:Safety issues of human iPSC-derived kidney organoids as a regenerative therapy need to be evaluated. Therefore, we studied the immunogenicity of human iPSC-derived kidney organoids. We subcutaneously implanted kidney organoids in immune-deficient IL2Ry-/-RAG2-/- mice for 1 month and hereafter performed adoptive transfer of healthy allogeneic human PBMC. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of kidney organoid cells and immune cell profiles. We investigated whether innate and adaptive immune cells invade kidney organoids, evoke an immune response, and influence the kidney organoid differentiation and functional capacity. Understanding the immunogenicity of kidney organoids will advance studies in the applicability of kidney organoids for regenerative medicine. Furthermore, it can serve as an in-vivo transplantation model to study solid organ transplantation.

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Human pluripotent stem cell-derived kidney organoids reveal tubular epithelial pathobiology of heterozygous HNF1B-associated dysplastic kidney malformations CL

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Human pluripotent stem cell-derived kidney organoids reveal tubular epithelial pathobiology of heterozygous HNF1B-associated dysplastic kidney malformations K

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