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The transcription of long non-coding RNAs is regulated by EGF in MCF10A cell line

ABSTRACT: The transcription of long non-coding RNAs is regulated by EGF in MCF10A cell line

PROVIDER: PRJEB18923 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1781671_1.fastq.gz	Fastqsanger.gz
	ERR1781671_2.fastq.gz	Fastqsanger.gz
	ERR1781672_1.fastq.gz	Fastqsanger.gz
	ERR1781672_2.fastq.gz	Fastqsanger.gz
	ERR1781673_1.fastq.gz	Fastqsanger.gz

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The transcription of long non-coding RNAs is regulated by EGF in MCF10A cell line

Project description:Whilst the biological function of many lncRNAs remains unknown, recent evidence has suggested that lncRNAs may be important regulators of cellular growth, differentiation and may play a significant role in cancer. Epidermal growth factor (EGF) an activator of the ERK1/2 signalling cascade is an important spatio-temporal regulator of transcription and, ultimately, of cellular growth and movement. In order to identify lncRNAs regulated by EGF signalling, we sequenced nuclear RNA in the presence or absence of EGF stimulation.

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Genome-wide analysis gene expression of MCF10A cells growing in the presence of EGF or AREG or without EGF or AREG for 24 hrs

Project description:Results of growing MCF10A cells continuously in serum free media supplemented with EGF (MCF10A) or AREG (MCF10A+AREG) followed by 24 hours of ligand withdrawl and measuring gene expression provides information as to what genes are regulated by AREG and EGF in a normal mammary epithelial cell model MCF10A cells continuously in serum free media supplemented with 10ng/ml of EGF (MCF10A) or 20ng/ml of AREG (MCF10A+AREG) followed by 24 hours of ligand withdrawl. Total RNA was collected and genome-wide analysis of expression was performed on RNA from each cell line.

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