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A new method for mithochondrial assembly

ABSTRACT: Assembly of a range of mitochondria across plants, fungi and animals to validate a new universal platome assembly methodology

PROVIDER: PRJEB19313 | ENA |

REPOSITORIES: ENA

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High concordance between SNPs identified by the porcine 60K BeadChip genotyping array (Illumina) and the assembly-versus-assembly method

2016-07-05 | GSE83910 | GEO

High concordance between SNPs identified by the porcine 60K BeadChip genotyping array (Illumina) and the assembly-versus-assembly method

Project description:Porcine 60K BeadChip genotyping arrays (Illumina) are increasingly being applied in pig genomics to validate SNPs identified by re-sequencing or assembly-versus-assembly method. Here we report that more than 98% SNPs identified from the porcine 60K BeadChip genotyping array (Illumina) were consistent with the SNPs identified from the assembly-based method. This result demonstrates that whole-genome de novo assembly is a reliable approach to deriving accurate maps of SNPs. To compare SNPs identified by genotyping arrays and de novo assembly method, we genotyped 10 pig breeds by porcine 60K BeadChip genotyping array (Illumina), including 1 berkshire pig, 1 hampshire pig, 1 landrace pig, 1 large white pig,1 piÃ©train pig, 1 bamei pig,1 jinhua pig, 1 meishan pig, 1 rongchang pig and 1 Tibetan wild boar.

2016-07-05 | E-GEOD-83910 | biostudies-arrayexpress

Metaproteomics on the digestive tract from Gammarus fossarum, an environmentally relevant sentinel.

Project description:Presentation of a new data treatment methodology for investigating the full range of biomasses and metabolic activities of the holobiont components from the digestive tract of Gammarus fossarum. Two tissues, intestine and hepatopancreatic caecum from individual animals were compared. Three animals have been analyzed in technical duplicates.

2021-09-09 | PXD016248 | Pride

BuTT-Seq: a new method for facile profiling of transcription

Project description:A wide range of sequencing methods have been developed to assess nascent RNA transcription and resolve the single-nucleotide position of RNA polymerase genome-wide. These techniques are often burdened with high input material requirements and lengthy protocols. We leveraged the template-switching properties of thermostable group II intron reverse transcriptase (TGIRT) and developed BuTT-Seq (BUlk analysis of nascent Transcript Termini sequencing), which can produce libraries from purified nascent RNA in 6 hours and from as few as 10,000 cells – an improvement of at least 10-fold over existing techniques. BuTT-Seq shows that inhibition of the superelongation complex (SEC) causes promoter-proximal pausing to move upstream in a fashion correlated with subnucleosomal fragments. To address transcriptional regulation in a tissue, BuTT-Seq was used to measure the circadian regulation of transcription from fly heads. All the results indicate that BuTT-Seq is a simple and powerful technique to analyze transcription at a high level of resolution.

2023-03-30 | GSE228595 | GEO

Mitochondrial Assembly in Sugarcane

Project description:Assembly of Sugarcane Mitochondria from Illumina and MinION data

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Project description:Quaternary range dynamics in alpine plants

| PRJNA528598 | ENA

Chitin oligosaccharide-responsive genes in suspension-cultured rice cells

Project description:Chitin is a major component of fungal cell walls and serves as a molecular pattern for the recognition of potential pathogens in the innate immune systems of both plants and animals. In plants, chitin oligosaccharides have been known to induce various defense responses in a wide range of plant cells including both monocots and dicots. We identified chitine oligosaccharide-responsive genes in suspension-cultured rice cells 1-12 h after treatment using rice 44k microarray.

2012-07-25 | GSE39633 | GEO

A new method to measure mechanics and dynamic assembly of branched actin networks.

Project description:We measured mechanical properties and dynamic assembly of actin networks with a new method based on magnetic microscopic cylinders. Dense actin networks are grown from the cylinders' surfaces using the biochemical Arp2/3-machinery at play in the lamellipodium extension and other force-generating processes in the cell. Under a homogenous magnetic field the magnetic cylinders self-assemble into chains in which forces are attractive and depend on the intensity of the magnetic field. We show that these forces, from piconewtons to nanonewtons, are large enough to slow down the assembly of dense actin networks and controlled enough to access to their non linear mechanical responses. Deformations are measured with nanometer-resolution, well below the optical resolution. Self-assembly of the magnetic particles into chains simplifies experiments and allows for parallel measurements. The combination of accuracy and good throughput of measurements results in a method with high potential for cell and cytoskeleton mechanics. Using this method, we observed in particular a strong non linear mechanical behavior of dense branched actin networks at low forces that has not been reported previously.

| S-EPMC5691053 | biostudies-literature

Chitin oligosaccharide elicitor-responsive genes in suspension cultured rice cells

2014-04-01 | GSE53414 | GEO

Nematode method calibration

Project description:Integrating quantitative morphological and qualitative molecular methods to analyze soil nematode community responses to plant range expansion

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