Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MiSeq 250 NGS raw data of WT-Env and T303-Env sequencing reads from microfluidic experiments.

ABSTRACT: High-throughput screening of neutralizing epitopes on HIV particles using single-virus droplet microfluidics

PROVIDER: PRJEB19490 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR1842940_1.fastq.gz	Fastqsanger.gz
	ERR1842940_2.fastq.gz	Fastqsanger.gz
	ERR1842950_1.fastq.gz	Fastqsanger.gz
	ERR1842950_2.fastq.gz	Fastqsanger.gz
	ERR1842941_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 28

Similar Datasets

MicDrop: Droplet Microfluidic Assay for Bacteria

Project description:High-throughput isolation and culture of human gut bacteria with droplet microfluidics

| PRJEB33065 | ENA

bacterial pathways involved in the catabolism of mucus glycans

Project description:Investigating host-microbiome interactions by droplet based microfluidics

| PRJEB32780 | ENA

Single nuclei RNA-seq using droplet microfluidics (DroNc-seq)

Project description:Single nuclei RNA-seq using droplet microfluidics (DroNc-seq)

| PRJNA413529 | ENA

A flexible microfluidic system for single-cell transcriptome profiling elucidates phased transcriptional regulators of cell cycle

Project description:Single cell or single nuclei RNA sequencing of mixed HEK 293 and 3T3 populations using the Nadia droplet sequencing platform. Samples cover different numbers of beads from which cDNA libraries were prepared.

2021-04-10 | E-MTAB-10296 | biostudies-arrayexpress

NanoLC-MSMS Phosphoproteomics comparison of in vitro cultured reticulocytes before and after passage through microfluidic capillaries

Project description:Triplicate samples (biological replicates) of in vitro culture-derived reticulocytes (derived from CD34+ cells) were analysed through qualitative phosphoproteomics before and after passage through a microfluidics capillary chip to investigate the mechanisms underlying shear stress recognition.

2019-09-12 | PXD013652 | Pride

Multiomics characterization of a less invasive microfluidic-based cell sorting technique

Project description:Fluorescence-activated cell sorting (FACS) is a specialized technique to isolate cell subpopulations with a high level of recovery and accuracy. However, the cell sorting procedure can impact the viability and metabolic state of cells. Here, we performed a comparative study and evaluated the impact of traditional high-pressure charged droplet-based and a microfluidic chip-based sorting approach on the metabolic and phosphoproteomic profile of different cell types. While microfluidic chip-based sorted cells more closely resembled the unsorted control group for most cell types tested, the droplet-based sorted cells showed significant metabolic and phosphoproteomic alterations. In particular, greater changes in redox and energy status were present in cells sorted with the droplet-based cell sorter along with higher transcriptional and spliceosomal regulation and mechanical stress signaling. These results indicate microfluidic chip-based sorting is less disruptive compared to droplet-based sorting.

2023-11-09 | MSV000093348 | MassIVE

spinDrop: a modular droplet microfluidic platform to maximise single-cell RNA sequencing information content

Project description:Droplet microfluidic methods have massively increased the throughput of single-cell RNA sequencing campaigns. The benefit of scale-up is, however, accompanied by increased background noise when processing challenging samples as well as lower overall RNA capture efficiency. These drawbacks stem from the lack of strategies to enrich for high-quality material at the moment of cell encapsulation and the lack of implementable multi-step enzymatic processes that increase RNA capture. Here we alleviate both bottlenecks by deploying fluorescence-activated droplet sorting to enrich for droplets that contain single viable cells, intact nuclei or fixed cells and use reagent addition to droplets by picoinjection to perform multi-step lysis and reverse transcription. Our methodology increases gene detection rates fivefold, while reducing background noise by up to half, depending on sample quality. We harness these unique properties to deliver a high-quality molecular atlas of mouse brain development using highly damaged input material. Our method is broadly applicable to other droplet-based workflows to deliver sensitive and accurate single-cell profiling at a reduced cost.

2023-07-02 | GSE208156 | GEO

Microfluidic device for single-molecule experiments with enhanced photostability.

Project description:A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel walls. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface-sample contacts and allows the in situ addition and combination of other reagents.

| S-EPMC2779842 | biostudies-literature

Pooled CRISPR screens with joint single-nucleus chromatin accessibility and transcriptome profiling

Project description:MultiPerturb-seq is a high-throughput CRISPR screening platform with joint single nucleus chromatin accessibility, transcriptome, and guide RNA capture. It uses combinatorial indexing combined with droplet microfluidics to scale throughput and integrate all three modalities. We apply MultiPerturb-seq to identify key genes whose loss can trigger differentiation in a rare pediatric cancer, atypical teratoid/rhabdoid tumor (AT/RT), which is driven by loss of the SWI/SNF chromatin remodeling subunit SMARCB1.

2024-09-22 | GSE277747 | GEO

Pooled CRISPR screens with joint single-nucleus chromatin accessibility and transcriptome profiling [CUT&RUN]

2024-10-13 | GSE278910 | GEO