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RNA-seq of E10.5 limb mesenchymal cells from mice with mutations in Mycn and mir17-92

ABSTRACT: RNA-seq of E10.5 limb mesenchymal cells from mice with mutations in Mycn and mir17-92

PROVIDER: PRJEB20972 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1989770.fastq.gz	Fastqsanger.gz
	ERR1989771.fastq.gz	Fastqsanger.gz
	ERR1989772.fastq.gz	Fastqsanger.gz
	ERR1989773.fastq.gz	Fastqsanger.gz
	ERR1989764.fastq.gz	Fastqsanger.gz

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RNA-seq of E10.5 limb mesenchymal cells from mice with mutations in Mycn and mir17-92

Project description:Mice with conditionally deactivating mutations in Mycn (Mycnfl/fl) or mir17-92 & mir106b (mir17-92fl/fl ; mir106b-/-) were crossed with mice expressing Cre-recombinase driven by the Prx1 promoter (Prx1Cre). Embryos were collected at E10.5, and distal compartment of hind- and fore-limbs were isolated from wild-type and conditional knock-out embryos (n=3 replicates per biologic group). Total RNA was extracted from each specimen with Trizol, and used in RNA-seq library preparation with Illumina Truseq mRNA library kit. Libraries were sequenced on the Illumina NextSeq system with 75bp single end reads.

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Chip-chip from Mouse E10.5 anterior and posterior immortalised limb cell lines and limb tissue for H3K27me3 antibody and from Mouse E10.5 anterior and posterior limb tissue for Ring1B antibody.

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