Project description:The uploaded dateset corresponds to MS1 and MS2 spectra obtained for Fc gamma receptor IIIb isolated from neutrophils of individual healthy donors. They represent NA1/NA2, NA2/NA2, NA2/NA2 allotype respectively. Based on the data, the targeted analysis of the N-glycosylation has been performed for the article: Wojcik et. al, Site-specific glycosylation mapping of Fc gamma receptor IIIb from neutrophils of individual healthy donors.
Project description:The ionic title complex, bis(mu-ethylene glycol)-kappa(3)O,O':O';kappa(3)O:O,O'-bis[(ethylene glycol-kappa(2)O,O')(ethylene glycol-kappaO)sodium] bis(ethylene glycolato-kappa(2)O,O')copper(II), [Na(2)(C(2)H(6)O(2))(6)][Cu(C(2)H(4)O(2))(2)], was obtained from a basic solution of CuCl(2) in ethylene glycol and consists of discrete ions interconnected by O-H...O hydrogen bonds. This is the first example of a disodium-ethylene glycol complex cation cluster. The cation lies about an inversion center and the Cu(II) atom of the anion lies on another independent inversion center.
Project description:Time series microarray analysis on the photosynthetic ciliate was conducted using an oligochip containing 15,654 genes designed from Teleaulax amphioxeia ESTs
Project description:Vascular endothelial cells (EC) and smooth muscle cells (SMC) require a decrease in cytoplasmic Ca2+ concentration after activation. This can be achieved by Ca2+ sequestration by the sarco-/endoplasmic reticulum Ca2+ pumps (SERCA) and Ca2+ extrusion by plasma membrane Ca2+ pumps (PMCA) and Na+-Ca2+-exchangers (NCX). Since the two cell types differ in their structure and function, we compared the activities of PMCA, NCX and SERCA in pig coronary artery EC and SMC, the types of isoforms expressed using RT-PCR, and their protein abundance using Western blots. The activity of NCX is higher in EC than in SMC but those of PMCA and SERCA is lower. Consistently, the protein abundance for NCX protein is higher in EC than in SMC and those of PMCA and SERCA is lower. Based on RT-PCR experiments, the types of RNA present are as follows: EC for PMCA1 while SMC for PMCA4 and PMCA1; EC for SERCA2 and SERCA3 and SMC for SERCA2. Both EC and SMC express NCX1 (mainly NCX1.3). PMCA, SERCA and NCX differ in their affinities for Ca2+ and regulation. Based on these observations and the literature, we conclude that the tightly regulated Ca2+ removal systems in SMC are consistent with the cyclical control of contractility of the filaments and those in EC are consistent with Ca2+ regulation of the endothelial nitric oxide synthase near the cell surface. The differences between EC and SMC should be considered in therapeutic interventions of cardiovascular diseases.
Project description:Sodium layered transition metal oxides have been considered as promising cathode materials for sodium ion batteries due to their large capacity and high operating voltage. However, mechanism investigations of chemical evolution and capacity failure at high voltage are inadequate. As a representative cathode, Na2/3Ni1/3Mn2/3O2, the capacity contribution at a 4.2 V plateau has long been assigned to the redox of the Ni3+/Ni4+ couple, while at the same time it suffers large irreversible capacity loss during the initial discharging process. In this work, we prove that the capacity at the 4.2 V plateau is contributed to the irreversible O2-/O2 n-/O2 evolution based on in situ differential electrochemical mass spectrometry and density functional theory calculation results. Besides, a phenomenon of oxygen release and subsequent surface lattice densification is observed, which is responsible for the large irreversible capacity loss during the initial cycle. Furthermore, the oxygen release is successfully suppressed by Fe substitution due to the formation of a unique Fe-(O-O) species, which effectively stabilizes the reversibility of the O2-/O2 n- redox at high operating voltage. Our findings provide a new understanding of the chemical evolution in layered transition metal oxides at high operating voltage. Increasing the covalency of the TM-O bond has been proven to be effective in suppressing the oxygen release and hence improving the electrochemical performance.