Chilli veinal mottle virus detection in Naga king chilli
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PROVIDER: PRJEB21757 | ENA |
REPOSITORIES: ENA
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Project description:Molecular characterisation of Capsicum chinense King Chilli for production of pungent and non pungent pigments
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Project description:Capsicum frutescens is one of the chilli varieties, with erect, highly pungent fruits. This variety is used to prepare hot sauces, chilli concentrates, and oleoresin. Its fruits are also used in traditional Indian medicine to treat arthritis and zoster related pain management. Infection by Polyphagotarsonemus latus commonly called as the Broad mite in chilli, make the leaves turn coppery and dark, show symptoms of leaf curl and stunted growth that eventually results in low yield and economic loss. We carried out gel-free, labelled mass spectrometry-based quantitative proteomic analysis of leaves and apical meristems of healthy and infected plants. We identified a total of 5799 proteins, of which total of which, 1677 proteins found to dysregulated in infested plants.
2020-08-31 | PXD018653 | Pride
Project description:The present study confirms the occurrence of Chilli veinal mottle virus (ChiVMV) under the genus Potyvirus in Naga chilli (Capsicum chinense) in Meghalaya based on mechanical transmission assay, transmission electron microscopy, RT-PCR and sequence analysis. This is the first record of Chivmv in Naga chilli in North-East India.
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Project description:Whole Transcriptome Sequencing of King Chilli from Manipur for identification of genes involved in biosynthesis of capsaicinoids and carotenoids
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Project description:Capsicum chinense leaves, primary metabolites, drought stress, quality control
| MSV000090332 | MassIVE
Project description:Capsicum spp. (hot peppers) demonstrate a range of interesting bioactive properties spanning anti-inflammatory, antioxidant, and antimicrobial activities. While several species within the genus are known to produce antimicrobial peptides (AMPs), AMP sequence mining of genomic data indicates this space remains largely unexplored. Herein, in silico AMP predictions are paired with peptidomics to identify novel AMPs from the interspecific hybrid ghost pepper (Capsicum chinense x frutescens). AMP prediction algorithms reveal 115 putative AMPs within the Capsicum chinense genome of which 14 were identified in the aerial tissue peptidome. PepSAVI-MS, de novo sequencing, and complementary approaches were used to fully molecularly characterize two novel AMPs, CC-AMP1 and CC-AMP2, including elucidation of post-translational modifications and disulfide bond connectivity. Both CC-AMP1 and CC-AMP2 have little homology with known AMPs and exhibit low µM antimicrobial activity against gram-negative bacteria including Escherichia coli and Klebsiella pneumoniae. These findings demonstrate the complementary nature of peptidomics, bioactivity-guided discovery, and bioinformatics-based investigations to more fully characterize plant AMP profiles.
2021-08-13 | PXD024605 | Pride
Project description:BackgroundChilli veinal mottle virus (ChiVMV), which belongs to the genus Potyvirus of the family Potyviridae, mainly infects solanaceous plants and has caused serious economic losses in Asia and Africa. Tobacco plants infected with ChiVMV suffered from punctate necrosis of leaves, leaf deformation, systemic necrosis of leaves and stems, and eventually plant death. However, ChiVMV infection could not usually be identified given the lack of rapid and efficient detection assays in tobacco plants. Therefore, an isolate of tobacco-infecting ChiVMV (ChiVMV-LZ) was obtained, and a novel isothermal amplification and detection technique, reverse transcription-recombinase polymerase amplification (RT-RPA), was established to detect ChiVMV in tobacco plants.MethodsIn this study, the full-length genome of ChiVMV-LZ was obtained using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) assays. The genome sequence of ChiVMV-LZ was characterized by sequence alignment and phylogenetic analysis. Then, a RT-RPA assay was established for rapid and sensitive detection of ChiVMV-LZ in tobacco. Additionally, the established RT-RPA assay was compared to the RT-PCR assay in aspect of sensitivity and application in field-collected tobacco samples.ResultsChiVMV-LZ was isolated from diseased tobacco in Luzhou, Sichuan, China. The tobacco plants inoculated with ChiVMV-LZ showed typical symptoms of yellow and round spots on the leaves, and curled and folded leaf margin, similar to those observed on naturally ChiVMV-infected tobacco in the field. The full-length genomic sequence of ChiVMV-LZ was determined to be 9742 nucleotides. Sequence alignment and phylogenetic analysis showed that ChiVMV-LZ was most closely related to ChiVMV-Yp8 isolated from pepper plants in Sichuan province while distantly related to ChiVMV-YN from tobacco in Yunnan province, indicating a possibly geographical differentiation of ChiVMV isolates. Additionally, a RT-RPA assay was established for rapid detection of ChiVMV in tobacco. The RT-RPA has no cross-reaction with other related tobacco viruses and is about 10-fold more sensitive than conventional RT-PCR method.ConclusionThe characterization of ChiVMV-LZ infecting tobacco was determined, and the established RT-RPA assay provides a reliable and effective method for rapid detection of ChiVMV in tobacco.
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