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Adhesion of FD phytoplasma VmpA to insect cells

ABSTRACT: The variable membrane protein VmpA of flavescence dorée phytoplasma acts as an adhesin binding cells of the insect vector Euscelidius variegatus

PROVIDER: PRJEB22700 | ENA |

REPOSITORIES: ENA

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A knockdown gene approach identifies an insect vector membrane protein with Leucin Rich Repeats as one of the receptors for the VmpA adhesin of flavescence dorée phytoplasma

Project description:The adhesion of flavescence dorée phytoplasma to the midgut epithelium cells of their insect vectors is partially mediated by the Variable Membrane Protein A (VmpA), an adhesin which shows lectin properties. In order to identify the insect receptor for VmpA, we lokked for Euscelidius variegatus cells proteins interacting with recombinant VmpA-His6 by mass spectrometry analysis of VmpA-E. variegatus protein complexes formed upon in vitro interaction assays.

2023-11-06 | PXD046447 | Pride

Grapevine comparative early transcriptome profiling reveals that Flavescence dorée phytoplasma represses plant responses induced by vector feeding in susceptible varieties

Project description:Flavescence dorée is the most serious grapevine yellows disease in Europe. It is caused by phytoplasmas which are transmitted from grapevine to grapevine by the leafhopper Scaphoideus titanus. Differences in susceptibility among grapevine varieties suggest the existence of specific genetic features associated with resistance to the phytoplasma and/or possibly with its vector. In this work, RNA-Seq was used to compare early transcriptional changes occurring during the three-trophic interaction between the phytoplasma, its vector and the grapevine, represented by two different cultivars, one very susceptible to the disease and the other scarcely susceptible. Background: Flavescence dorée is the most serious grapevine yellows disease in Europe. It is caused by phytoplasmas which are transmitted from grapevine to grapevine by the leafhopper Scaphoideus titanus. Differences in susceptibility among grapevine varieties suggest the existence of specific genetic features associated with resistance to the phytoplasma and/or possibly with its vector. In this work, RNA-Seq was used to compare early transcriptional changes occurring during the three-trophic interaction between the phytoplasma, its vector and the grapevine, represented by two different cultivars, one very susceptible to the disease and the other scarcely susceptible. The comparison of the transcriptomic responses highlighted both passive and active defense mechanisms against the vector and/or the pathogen in the scarcely-susceptible variety, as well as the capacity of the phytoplasmas to repress the defense reaction against the insect in the susceptible variety.

2019-06-13 | E-MTAB-7710 | biostudies-arrayexpress

FTSH genes of Flavescence doree phytoplasma

Project description:Flavescence dorée phytoplasma has multiple ftsH genes differentially expressed in plants and insects

| PRJEB26323 | ENA

RNAseq of Flavescence dorée phytoplasma infecting grapevine leaves

Project description:A comprehensive transcriptional landscape of Flavescence dorée phytoplasma infecting in field-grown Vitis vinifera leaves

| PRJEB6982 | ENA

Flavescence doree phytoplasma strain FD92 gene for variable membrane protein B

Project description:Flavescence doree phytoplasma strain FD92 gene for variable membrane protein B

| PRJEB32304 | ENA

Project description:Molecular imprinting in grapevines recovered from Flavescence dorée phytoplasma

| PRJNA580028 | ENA

phytoplasma in plants vs. phytoplasma in insects (co-hybridizations)

Project description:Transcriptional profiling of phytoplasma grown in plant (Chrysanthemum coronarium) and grown in insect (Macrosteles striifrons). Two-condition experiment, phytoplasma-infected plant and phytoplasma-infected insect. Biological replicates: 6 phytoplasma-infected plants and 6 phytoplasma-infected insects, independently grown and harvested. One replicate per array.

2012-01-16 | E-GEOD-30302 | biostudies-arrayexpress

phytoplasma in plants vs. phytoplasma in insects (co-hybridizations)

Project description:Transcriptional profiling of phytoplasma grown in plant (Chrysanthemum coronarium) and grown in insect (Macrosteles striifrons).

2012-01-16 | GSE30302 | GEO

phytoplasma in plants vs. phytoplasma in insects (single hyb expts)

Project description:Transcriptional profiling of phytoplasma grown in plant (Chrysanthemum coronarium) and grown in insect (Macrosteles striifrons). Two-condition experiment, phytoplasma-infected plant and phytoplasma-infected insect. Biological replicates: 4 phytoplasma-infected plants and 4 phytoplasma-infected insects, independently grown and harvested. One replicate per array.

2012-01-16 | E-GEOD-29274 | biostudies-arrayexpress

phytoplasma in plants vs. phytoplasma in insects (single hyb expts)

Project description:Transcriptional profiling of phytoplasma grown in plant (Chrysanthemum coronarium) and grown in insect (Macrosteles striifrons).

2012-01-16 | GSE29274 | GEO

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