Transcriptome of Thorellius atrox scorpion by RNA-seq
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ABSTRACT:
PROVIDER: PRJEB23004 | ENA |
REPOSITORIES: ENA
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Project description:The clinical manifestations of Bothrops atrox envenoming involve local and systemic changes, among which, edema requires substantial attention due to its ability to progress to compartmental syndromes, and sometimes, causing tissue loss and amputations. Thus, the present study aimed to explore the systemic pathological and inflammatory events that are altered by intraplantar injection of B. atrox venom in mice. Plasma samples collected showed differential abundance proteins related to complement, coagulation, lipid system, platelet and neutrophil degranulation and pathways related to cell death and ischemic tolerance. B. atrox venom can induces a different pattern of blood proteome alterations of mice according to the severity of the edema.
2022-05-10 | MSV000089455 | MassIVE
Project description:Background: Bothrops atrox is known to be the pitviper responsible for most snakebites and human fatalities in the Amazon region. It can be found in a wide geographical area including the northern South America, the east of Andes and the Amazon basin. Possibly due to its wide distribution range and generalist feeding, intraspecific venom variation was reported by previous proteomics studies. Sex-based and ontogenetic variations on venom compositions of Bothrops snakes were also subject of proteomic and peptidomic analysis. However, the venom peptidome of B. atrox remains unknown. Methods: we conducted a mass spectrometry-based analysis of the venom peptides of individual male and female specimens combining bottom-up and top-down approaches. Results: We identified in B. atrox a total of 105 native peptides in the mass range of 0.4 to 13.9 kDa. Quantitative analysis showed that Phospholipase A2 and Bradykinin Potentiating Peptides were the most abundant peptide families in both genders, but the disintegrins levels were significantly increased in the venoms of females. Known peptides processed at non-canonical sites and new peptides were also revealed in this work. Conclusion: The venom peptidomes of male and female specimens of B. atrox were analyzed by mass spectrometry-based approaches in this work. The study points to differences in the disintegrin levels in the venoms of females that may result in distinct pathophysiology in envenomation. Further research is needed to explore its biological implications.
2020-10-13 | PXD018632 | Pride
Project description:proteomic analysis of venom gland peptides from the scorpion Centruroides vittatus
2022-02-15 | PXD019850 | Pride
Project description:Diachasmimorpha longicaudata parasitoid wasps carry a symbiotic poxvirus, known as DlEPV, within the female wasp venom gland. We sequenced RNA from venom gland tissue to identify DlEPV orthologs for 3 conserved poxvirus core genes. The DlEPV ORFs identified from this transcriptome were used to design primers for downstream RT-qPCR analysis and RNAi knockdown experiments.
2020-02-03 | GSE144541 | GEO