Rosa chinensis cv 'Old Blush' genome
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB24406 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Rosa chinensis ‘Pallida’ (Rosa L.) is one of the most important ancient rose cultivars originating from China. It contributed the ‘tea scent’ trait to modern roses. However, little information is available on the gene regulatory networks involved in scent biosynthesis and metabolism in Rosa. In this study, the transcriptome of R. chinensis ‘Pallida’ petals at different developmental stages, from flower buds to senescent flowers, was investigated using Illumina sequencing technology. De novo assembly generated 89,614 clusters with an average length of 428 bp. Based on sequence similarity search with known proteins, 62.9% of total clusters were annotated. Out of these annotated transcripts, 25,705 and 37,159 sequences were assigned to gene ontology and clusters of orthologous groups, respectively. The dataset provides information on transcripts putatively associated with known scent metabolic pathways. Digital gene expression (DGE) was obtained using RNA samples from flower bud, open flower and senescent flower stages. Comparative DGE and quantitative real time PCR permitted the identification of five transcripts encoding proteins putatively associated with scent biosynthesis in roses. The study provides a foundation for scent-related genes discovery in roses.
2015-01-01 | GSE54486 | GEO
Project description:Rosa chinensis cultivar:Old Blush Transcriptome or Gene expression
| PRJNA351281 | ENA
Project description:Rosa chinensis cultivar:Old Blush Genome sequencing and assembly
| PRJNA413292 | ENA
Project description:RcMYB84 and RcMYB123 synergistically mediate JA induced defense against Botrytis cinerea in Rosa chinensis
| PRJNA606918 | ENA
Project description:RNAseq raw sequence reads of rose petals from Rosa chinensis cv. ‘Old Blush’, Rosa x wichurana and their 2 progenies
| PRJEB37774 | ENA
Project description:The floral transition is a crucial developmental event, but little is known about the underlying regulatory networks in seasonally and continuously flowering roses. In this study, we compared the genetic basis of flowering in two rose species, Rosa chinensis 'Old Blush', which flowers continuously, and R. odorata var. gigantea, which blooms in early spring. Gene ontology (GO) terms related to methylation, light reaction, and starch metabolism were enriched in R. odorata var. gigantea and terms associated with sugar metabolism were enriched in R. chinensis 'Old Blush' during the floral transition. A MapMan analysis revealed that genes involved in hormone signaling mediate the floral transition in both taxa. Furthermore, differentially expressed genes (DEGs) involved in vernalization, photoperiod, gibberellin (GA), and starch metabolism pathways converged on integrators, e.g., LFY, AGL24, SOC1, CAL, and COLs, to regulate the floral transition in R. odorata var. gigantea, while DEGs related to photoperiod, sugar metabolism, and GA pathways, including COL16, LFY, AGL11, 6PGDH, GASA4, and BAM, modulated the floral transition in R. chinensis 'Old Blush.' Our analysis of the genes underlying the floral transition in roses with different patterns of flowering provides a basis for further functional studies.
| S-EPMC5519770 | biostudies-literature
Project description:Petal expansion is the main process by which flower opening occurs in roses (Rosa chinensis). Although the regulation of leaf expansion has been extensively studied, little is known about the mechanisms controlling petal expansion. The regulation of leaf dorsoventral (adaxial-abaxial) polarity is important for blade expansion and morphogenesis, but the mechanisms involved adaxial-abaxial regulation in petals are unknown. We found that auxin, a key hormonal regulator of leaf adaxial-abaxial patterning, is unevenly distributed in rose petals. The transcriptomes of the adaxial and abaxial petal tissues were sequenced at three developmental stages during flower opening. Genes that were differentially expressed between the two tissues were filtered for those known to be involved in petal expansion and phytohormone biosynthesis, transport, and signaling, revealing potential roles in petal expansion, especially auxin pathway genes. Using a weighted gene coexpression network analysis (WGCNA), we identified two gene modules that may involve in adaxial-abaxial regulation, 21 and five hub genes have been found respectively. The qRT-PCR validation results were consistent with the RNA-seq data. Based on these findings, we propose a simple network of adaxial-abaxial-related genes that regulates petal expansion in R. chinensis "Old Blush." For the first time, we report the adaxial-abaxial transcriptional changes that occur during petal expansion, providing a reference for the study of the regulation of polarity in plant development.
| S-EPMC6747050 | biostudies-literature
Project description:Rosa chinensis breed:Rosa chinensis Raw sequence reads
| PRJNA905852 | ENA
Project description:affy_cinetique_lyon_rose: Flower/Petal initiation and development in roses
| PRJNA118013 | ENA