Endophytes of mangroves
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ABSTRACT:
PROVIDER: PRJEB24543 | ENA |
REPOSITORIES: ENA
Similar Datasets
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Project description:Two potato cultivars, Russet Burbank and Bionta, were inoculated with three different endophytes containing different AHL types. The impact of the endophytes to the different cultivars was measured by gene expression analysis with a customized microarray B. phytofirmans type strain PsJN was originally isolated as a contaminant from surface-sterilized, Glomus vesculiferum-infected onion roots (Nowak et al., 1998), whereas strain P6 RG6-12 was isolated from the rhizosphere of a grassland in the Netherlands (Salles et al., 2006). This strain was selected based on its similarity to strain PsJN based on 16S rRNA gene homology, and similar phenotypic features. Both strains were generally cultivated on King's medium (King et al., 1954). For the mutant AHL to the strain B. phytofirmans PsJN a quorum quenching approach as described by Wopperer et al., 2006 was employed. Plasmid pMLBAD-aiiA, which contains aiiA, the Bacillus sp. 240B1 lactonase gene, was transferred to B. phytofirmans PsJN by triparental mating as described by de Lorenzo and Timmis (1994). 2 cultivars, 3 endophytes
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Project description:MicroRNAs (miRNAs) and endogenous small interfering RNAs (siRNAs) play an important role in plant stress responses but the small RNA (sRNA) repertoire is poorly characterized in extremphophiles. We report sRNA transcriptomes of leaf and flower tissues of two mangrove species Bruguiera gymnorrhiza and Kandelia candel (Rhizophoraceae) based on Illumina sequencing. A total of 32 known and 5 novel miRNA families were identified collectively.Besides the conserved miR390-TAS3-ARF pathway, B. gymnorrhiza possessed additional high-abundance tasiRNAs derived from the less-conserved regions of TAS3 transcripts with expanded potential targets.his study provides the first comprehensive sRNA transcriptome for mangroves B. gymnorrhiza and K. candel. Comparative analyses revealed that these two species systematically optimize sRNA expression levels, accompanied with rewiring of sRNA regulatory networks, as an evolutionarily strategy for stress adaptations. Furthermore, 24-nt sRNAs mapped to the telomeric repeats (CCCTAAA)n were highly abundant in both B. gymnorrhiza and K. candel.
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