Project description:Transcriptional profiling of Pinus pinaster adult needles in a complete year. The needles were isolated by year emergence (whorl) in four groups. 0 (2012), 1 (2011), 2 (2010) and 3 (2009). The samples were harvested at 1245 m of altitude at Los Reales de Sierra Bermeja (Spain) (30S X:303.095 Y:4.039.618) one time per month.
Project description:Transcriptional profiling of Pinus pinaster adult needles in a complete year. The needles were isolated by year emergence (whorl) in four groups. 0 (2012), 1 (2011), 2 (2010) and 3 (2009). The samples were harvested at 1245 m of altitude at Los Reales de Sierra Bermeja (Spain) (30S X:303.095 Y:4.039.618) one time per month. Reference design. 6 biological replicates that were pooled two by two resulting in 3 samples for the hybridization. The common reference samples was a pool of RNA samples from January. The reference sample was labeled with Cy5 and the test samples with Cy3.
Project description:Vanee2010 - Genome-scale metabolic model of
Cryptosporidium hominis (iNV213)
This model is described in the article:
A genome-scale metabolic
model of Cryptosporidium hominis.
Vanee N, Roberts SB, Fong SS, Manque
P, Buck GA.
Chem. Biodivers. 2010 May; 7(5):
1026-1039
Abstract:
The apicomplexan Cryptosporidium is a protozoan parasite of
humans and other mammals. Cryptosporidium species cause acute
gastroenteritis and diarrheal disease in healthy humans and
animals, and cause life-threatening infection in
immunocompromised individuals such as people with AIDS. The
parasite has a one-host life cycle and commonly invades
intestinal epithelial cells. The current genome annotation of
C. hominis, the most serious human pathogen, predicts 3884
genes of which ca. 1581 have predicted functional annotations.
Using a combination of bioinformatics analysis, biochemical
evidence, and high-throughput data, we have constructed a
genome-scale metabolic model of C. hominis. The model is
comprised of 213 gene-associated enzymes involved in 540
reactions among the major metabolic pathways and provides a
link between the genotype and the phenotype of the organism,
making it possible to study and predict behavior based upon
genome content. This model was also used to analyze the two
life stages of the parasite by integrating the stage-specific
proteomic data for oocyst and sporozoite stages. Overall, this
model provides a computational framework to systematically
study and analyze various functional behaviors of C. hominis
with respect to its life cycle and pathogenicity.
This model is hosted on
BioModels Database
and identified by:
MODEL1507180071.
To cite BioModels Database, please use:
BioModels Database:
An enhanced, curated and annotated resource for published
quantitative kinetic models.
To the extent possible under law, all copyright and related or
neighbouring rights to this encoded model have been dedicated to
the public domain worldwide. Please refer to
CC0
Public Domain Dedication for more information.
Project description:Vibrio alginolyticus is a Gram-negative marine bacterium. A limited population of the organisms causes acute gastroenteritis in humans. In this study, Vibrio alginolyticus wild type strain EPGS is compared with the mutants of Ser-Thr kinase PpkA and phosphatase PppA, after cultured for 7h, in Luria-Bertani containing medium 3 % NaCl at 30 C. Our goal is to determine the ppkA and pppA regulon.
Project description:A total of 52 patients were analyzed: 21 of them monoinfected with HCV and 31 coinfected with HIV (HCV/HIV). HCV patients were recruited from Hospital Italiano and Hospital José María Ramos Mejía from Buenos Aires, Argentina, and HCV/HIV patients from Hospital Universitario La Paz, Hospital Infanta Leonor, Hospital Universitario La Princesa, Hospital Puerta de Hierro, from Madrid, Spain. All samples were processed at the National Center for Microbiology (Madrid). Patients were naıve of treatment for HCV. CHC infection was defined by the presence of anti-HCV antibodies in serum and detectable HCV RNA in plasma samples in at least 2 separate occasions. All HIV+ patients had HIV antibodies, CD4+ T-cells counts ≥ 500 cel/mm3 for at least one year before sample collection, and undetectable HIV viral load since they received suppressive antiretroviral treatment (ART) for at least one year. Plasma extracellular vesicles isolation and RNA purification was performed using the ExoRNeasy Serum/Plasma Midi kit (QIAGEN, Cat #77044). EVs were phenol-lysed and total RNA was purified by ethanol-based membrane binding into spin columns. Quality and integrity were evaluated by the Bioanalyzer 2100 with Agilent RNA 6000 Nano kit (Agilent). Small RNA library synthesis and sequencing were performed at Centre for Genomic Regulation (CRG) at Barcelona (Spain). Small RNA libraries were constructed with Illumina’s TruSeq Small RNA kit v.4 (Illumina) and 50nts (1x50) were sequenced in an Illumina HiSeq2500, with a single read approach.