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RNA-seq of IMR-5/75 neuroblastoma cell line upon TBX2 and/or MYCN knockdown

ABSTRACT: RNA-seq of IMR-5/75 neuroblastoma cell line upon TBX2 and/or MYCN knockdown

PROVIDER: PRJEB25334 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2360080.fastq.gz	Fastqsanger.gz
	ERR2360090.fastq.gz	Fastqsanger.gz
	ERR2360100.fastq.gz	Fastqsanger.gz
	ERR2360110.fastq.gz	Fastqsanger.gz
	ERR2360120.fastq.gz	Fastqsanger.gz

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RNA-seq of IMR-5/75 neuroblastoma cell line upon TBX2 and/or MYCN knockdown

Project description:RNA-seq upon TBX2, MYCN or combination of TBX2 and MYCN knockdown in the neuroblastoma cell line IMR-5/75. Cells were transduced with two different shRNAs (shTBX2_2 and shTBX2_4) targeting TBX2 and a non-targeting control (NTC), and selected with puromycin. Cells were treated with doxycycline for shMYCN induction (with DOX or not). Analysis was performed three days upon TBX2 knockdown and two days upon MYCN knockdown, including six biological replicates per condition.

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Project description:Inducible MYCN-knockdown, followed by RNA-seq analysis in the MYCN-amplified neuroblastoma cell line IMR5-75, reveals profound time-dependent transcriptome changes. For modulation of MYCN levels, stable neuroblastoma cell models were used where MYCN can be downregulated via vector-based hairpin RNA induction upon addition of 1Âµg/ml tetracycline (IMR5-75-shMYCN. From cells treated either with tetracycline or solvent (ethanol), RNA was isolated at time points 6 hours, 12 hours and 24 hours. Experiments were done in duplicates. RNA was sequenced.

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Global transcriptome analysis in the MYCN-amplified neuroblastoma cell line IMR5-75 upon inducible MYCN-knockdown

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Project description:ChIP-seq of neuroblastoma IMR-32, CLB-GA, NGP, IMR-5/75, GI-M-EN, CHP-212, and IMR-5 cell lines to identify TBX2, MYCN, H3K27ac, H3K4me1 and H3K4me3 binding sites

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NB IMR-5/75 medium perturbation

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The Super Elongation Complex Drives Transcriptional Addiction in MYCN-amplified Neuroblastoma

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