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RNA-seq of barley lines after dehydration, spider mite or combined stress.

ABSTRACT: RNA-seq of barley lines after dehydration, spider mite or combined stress.

PROVIDER: PRJEB25386 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR2365610.fastq.gz	Fastqsanger.gz
	ERR2365611.fastq.gz	Fastqsanger.gz
	ERR2365612.fastq.gz	Fastqsanger.gz
	ERR2365613.fastq.gz	Fastqsanger.gz
	ERR2365604.fastq.gz	Fastqsanger.gz

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RNA-seq of barley lines after dehydration, spider mite or combined stress.

Project description:Drought and herbivores are main threats to crop production. Barley plants were subjected to dehydration, spider mite attack or to a combination of both stresses. RNA-seq analyses were done to know how individual and double abiotic-biotic stresses promote changes in the transcriptome.

2018-04-30 | E-MTAB-6565 | biostudies-arrayexpress

Barley transcriptional responses to specialist and generalist spider mite herbivores

Project description:Spider mites, including the two-spotted spider mite (Tetranychus urticae, TSSM) and the Banks grass mite (Oligonychus pratensis, BGM), are becoming increasingly important agricultural pests. The TSSM is an extreme generalist documented to feed on more than 1100 plant hosts. In contrast, the BGM is a grass specialist, with hosts including important cereal crops like maize, wheat, sorghum and barley. Historically, studies of plant-herbivore interactions have focused largely on insects. However, far less is known about plant responses to spider mite herbivores, especially in grasses, and whether responses differ between generalists and specialists. To identify plant defense pathways responding to spider mites, we collected time course RNA-seq data from barley (Hordeum vulgare L.) infested with TSSMs and BGMs. Additionally, and as a comparison to the physical damage caused by spider mite feeding, a wounding treatment was also included.

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Time course RNA-seq data from barley infested with Tetranychus urticae and Banks grass mite

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Project description:Spider mites, including the two-spotted spider mite (Tetranychus urticae, TSSM) and the Banks grass mite (Oligonychus pratensis, BGM), are becoming increasingly important agricultural pests. The TSSM is an extreme generalist documented to feed on more than 1100 plant hosts. In contrast, the BGM is a grass specialist, with hosts including important cereal crops like maize, wheat, and sorghum. Historically, studies of plant-herbivore interactions have focused largely on insects. As such, far less is known about plant responses to spider mite herbivores, especially in grasses, and whether responses differ between generalist and specialist mites. To identify plant defense pathways responding to spider mites, we collected time course RNA-seq data from maize (Zea mays) infested with TSSMs and BGMs. Additionally, and as a comparison to the physical damage caused by spider mite feeding, a wounding treatment was also included. In total, four biological samples were generated per treatment.

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Project description:We sequenced messenger RNA from mixed stages of the two-spotted spider mite (Tetranychus urticae) reared on bean (Phaseolus vulgaris cv California Red Kidney; the laboratory host plant for mites) and two Arabidopsis thaliana accessions which were considered to either be susceptible (Kondara) or resistant (Bla-2) to mite feeding. This pilot experiment was conducted to assess gene expression differences of mites grown on sensitive versus resistant Arabidopsis accessions, as well as differences in mites feeding on different host species. The expression data was used for gene model validation of genes predicted by EuGene in the spider mite genome and to assess gene expression levels. Examination of gene expression of spider mites reared on beans and two Arabidopsis accessions (Kondara and Bla-2).

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