Project description:Amplification of glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) partial sequences from promastigote clones of Brazilian clinical isolates of Trypanosomatidae sp.
Project description:Methylation differences between SIC 5 cocoa seedlings germinated in a common environment before growth for 74 days under semic controlled environmental conditions set to mimic the Malaysian or Brazilian temperature environment. Genomic DNA sonicated and the methylated fraction enriched using a 5-methylcytosine antibody before whole genome amplification and hybridisation to the array. Two-condition experiment, Methyl enriched gDNA Malaysian vs. Brazilian leaf samples. 7 Biological replicates independently grown and harvested. Environmental (temperature) effect
Project description:Leishmania infantum (Kinetoplastida:Trypanosomatidae) is the etiological agent of zoonotic visceral leishmaniasis in the Mediterranean basin. The motile promastigote stage infects the hematophagous sand fly vector host and amastigotes survives and multiplies within phagocytes of the mammalian host. Promastigotes are routinely cultured in liquid undefined media and are considered to mimic the environment within the sand fly gut. We have put this to the test by high-throughput gene expression profiling by shotgun DNA microarrays generated in our laboratory. This has been possible thanks to RNA amplification.
