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Quantitative RNA-seq analysis of the transcriptome of Campylobacter jejuni

ABSTRACT: Quantitative RNA-seq analysis of the transcriptome of Campylobacter jejuni

PROVIDER: PRJEB2577 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR036497.fastq.gz	Fastqsanger.gz
	ERR036498.fastq.gz	Fastqsanger.gz
	ERR036499.fastq.gz	Fastqsanger.gz
	ERR036500.fastq.gz	Fastqsanger.gz

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[E-MTAB-706] Quantitative RNA-seq analysis of the transcriptome of Campylobacter jejuni

Project description:RNA-seq analysis of the transcriptome of wild type C.jejuni NCTC11168, and of an rpoN mutant of the same strain, both grown in vitro. ArrayExpress Release Date: 2011-06-14 Publication Title: Quantitative RNA-seq analysis of the transcriptome of Campylobacter jejuni Publication Author List: Roy R. Chaudhuri, Lu Yu, Alpa Kanji, Timothy T. Perkins, Paul P. Gardner, Jyoti Choudhary, Duncan J. Maskell, Andrew J. Grant Person Roles: submitter Person Last Name: Chaudhuri Person First Name: Roy Person Mid Initials: R Person Email: roy.chaudhuri@gmail.com Person Phone: 441000000000 Person Address: Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, United Kingdom Person Affiliation: University of Cambridge

2011-07-14 | GSE30621 | GEO

Quantitative RNA-seq analysis of the Campylobacter jejuni transcriptome.

Project description:Campylobacter jejuni is the most common bacterial cause of foodborne disease in the developed world. Its general physiology and biochemistry, as well as the mechanisms enabling it to colonize and cause disease in various hosts, are not well understood, and new approaches are required to understand its basic biology. High-throughput sequencing technologies provide unprecedented opportunities for functional genomic research. Recent studies have shown that direct Illumina sequencing of cDNA (RNA-seq) is a useful technique for the quantitative and qualitative examination of transcriptomes. In this study we report RNA-seq analyses of the transcriptomes of C. jejuni (NCTC11168) and its rpoN mutant. This has allowed the identification of hitherto unknown transcriptional units, and further defines the regulon that is dependent on rpoN for expression. The analysis of the NCTC11168 transcriptome was supplemented by additional proteomic analysis using liquid chromatography-MS. The transcriptomic and proteomic datasets represent an important resource for the Campylobacter research community.

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