OsbZIP73 Chip-Seq
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ABSTRACT:
PROVIDER: PRJEB26085 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:ChIP-seq analysis of bZIP73 binding sites in rice seedlings (Zhonghua11)
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Project description:To identify genome-wide binding sites of MADS27, we performed ChIP-seq experiment from 3xMyc-OsMADS27OE rice seedlings.
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Project description:IDS1 is a rice AP2-type transcription factor with transcritpional repression activity. To understand how IDS1 regulate rice salt tolerance, the ChIP-seq experiments were performed to identify IDS1 binding site in globle genomic level. The two-weeks-old rice seedlings were lysated and sonificated and IDS1-DNA complexes were immune precipated with myc-antibody and protein A beads. The purified DNA samples were used to construct sequencing libraries and sequenced with Illumina. The data were then analyzed with bio-informatic tools.
2018-04-30 | E-MTAB-6504 | biostudies-arrayexpress
Project description:As a species mostly planted in tropical and subtropical regions, rice is sensitive to chilling temperature, especially at reproductive stage. However, the effect of low temperature on seed development has not been well characterized. The transcriptome of two rice cultivars Zhonghua11 and Hanfeng were analyzed to characterize the gene regulatory networks of rice seed during low temperature treatment. Whole plants of two rice cultivars Zhonghua11 (low temperature sensitive) and Hanfeng (low temperature tolerance) were treated at 14°C for 2 days during seed development stage. The plants without treatment serve as controls. Rice seeds were harvested for RNA extraction.
2012-02-19 | E-GEOD-31077 | biostudies-arrayexpress
Project description:To reveal the underlying molecular mechanism of Gif1 action in the control of grain filling and yield improvement, we performed transcriptional profiling of wild type Zhonghua11 and mutant gif1 plants in early filling stage on a global scale using the Affymetrix GeneChip Rice Genome Array Experiment Overall Design: Rice caryopsis were harvested in 7 days after flowering and three biological repeats were performed on Zhonghua11 (wild-type) and gif1 (mutant), respectively.
2008-11-06 | E-GEOD-9498 | biostudies-arrayexpress
Project description:Gene expression is controlled by the complex interaction of transcription factors binding to promoters and other regulatory DNA elements. One common characteristic of the genomic regions associated with regulatory proteins is a pronounced sensitivity to DNase I digestion. We generated genome-wide high resolution maps of DNase I hypersensitive (DH) sites from both seedling and callus tissues of rice. Approximately 25% of the DH sites from both tissues were found in the putative promoters, indicating that the vast majority of gene regulatory elements in rice are not located at promoter regions. We found 58% more DH sites in callus than in seedling. For DH sites detected in both seedling and callus, 31% displayed significantly different levels of DNase I sensitivity within the two tissues. Genes that were differentially expressed in seedling and callus were frequently associated with DH sites in both tissues. The DNA sequences contained within the DH sites were hypomethylated, consistent with what is known about active gene regulatory elements. Interestingly, tissue-specific DH sites located in the promoters showed an elevated level of DNA methylation. A distinct elevation of H3K27me3 was associated with intergenic DH sites. These results suggest that epigenetic modifications play a role in the dynamic changes of the numbers and DNase I sensitivity of DH sites during development. To do associated analysis with DNase I hypersensitive sites in rice, we performed ChIP-seq to identify the positions of three histone modifications (H3K4me2, H3K36me3 and H4K12ac) in the rice genome (leaf tissue only - not callus). The ChIP DNA from seedling of each experiment was sequenced on one lane of Illumina Genome Analyzer.
2011-11-09 | E-GEOD-26733 | biostudies-arrayexpress
Project description:As a species mostly planted in tropical and subtropical regions, rice is sensitive to chilling temperature, especially at reproductive stage. However, the effect of low temperature on seed development has not been well characterized. The transcriptome of two rice cultivars Zhonghua11 and Hanfeng were analyzed to characterize the gene regulatory networks of rice seed during low temperature treatment.
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Project description:Comparison of the binding of GOLDEN2-LIKE (GLK) transcription factors in tomato, tobacco, Arabidopsis, maize and rice, show that genome cis-variation caused wide-spread TF binding divergence, and most of the TF binding sites are genetically redundant.
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Project description:The angle of rice leaf inclination is an important agronomic trait and closely related to the yield and architecture of crops. Through genetic screening, a rice gain-of-function mutant leaf inclination1, lc1, was identified . Phenotypic analysis confirmed the exaggerated leaf angels of lc1 due to the stimulated cell elongation at the collar.In this series, we compare the transcriptome of zhonghua11 and lc1 collar.
2011-06-16 | GSE16640 | GEO