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RNA Seq of colonic Lgr5+ epithelial stem cells in Il22+/+ and Il22-/- mice during homeostasis and 24h after irradiation with 8 Gy

ABSTRACT: RNA Seq of colonic Lgr5+ epithelial stem cells in Il22+/+ and Il22-/- mice during homeostasis and 24h after irradiation with 8 Gy

PROVIDER: PRJEB26459 | ENA |

REPOSITORIES: ENA

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RNA Seq of colonic Lgr5+ epithelial stem cells in Il22+/+ and Il22-/- mice during homeostasis and 24h after irradiation with 8 Gy

Project description:To investigate the influence of Interleukin-22 (IL-22) on colonic intestinal stem cells, we assessed gene expression in these cells during homeostasis and after induction of DNA damage. IL-22 is a lymphocyte-derived cytokine that targets exclusively non-hematopoietic cells. The receptor is expressed on intestinal epithelial cells, including Lgr5+ stem cells. The colonic Lgr5+ epithelial stem cells were highly purified as DAPI-EpCam+CD45-CD24MedLgr5+. DNA damage was induced by whole body irradiation with 8 Gy and cells were isolated 24h after exposure. The following populations were analyzed: Wildtype, unirradiated (Ctrl) Il22-/-, unirradiated (Ctrl) Wildtype, 24h after 8Gy Il22-/-, 24h after 8Gy

2019-01-01 | E-MTAB-6723 | biostudies-arrayexpress

6 Gy gamma-irradiation of mouse small intestinal organoids at 24h, 48h and 96h after irradiation

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6 Gy gamma-irradiation of mouse small intestinal organoids at 24h, 48h and 96h after irradiation

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transcriptomic assay on embryos after barley seeds gamma_irradiation-Molecular mechanisms of growth stimulation after low-dose irradiation of barley seeds

Project description:4plex_barley_2017_01 - transcriptomic assay on embryos after barley seeds gamma_irradiation - Why radiation exposure on seeds at low doses causes stimulation of plant growth after germination? - Barley dry seeds of variety “Nur” were irradiated by gamma-rays (Co-60) at doses 20 Gy (stimulatory) and 100 Gy (inhibitory). Immediately after irradiation we put seeds for germination to Petri dishes containing water filter paper; embryos were taken 2h after irradiation, embryos with embrionic roots were taken 24 and 48h after irradiation. The following comparisons have been made: non-irradiated (0 Gy), 2h vs. 20 Gy, 2h; 0 Gy, 24h vs. 20 Gy, 24h; 0 Gy, 48h vs. 20 Gy, 48h; 0 Gy, 24h vs. 100 Gy, 24h. Three independent biological replicates were used.

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cDNA array 6800 genes (NIB) - HaCaT J10 24h - 0.5&2 Gy

Project description:Human HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye-swap hybridization procedure 24 h after irradiation. Keywords = human keratinocytes, gamma irrdiation Keywords: dose response

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E-MTAB-6723

Project description:RNA Seq of colonic Lgr5+ epithelial stem cells in Il22+/+ and Il22-/- mice during homeostasis and 24h after irradiation with 8 Gy

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