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SynSTARR-seq with Glucocorticoid Receptor synthetic binding sites in GR18 cell line

ABSTRACT: synSTARR-seq with Glucocorticoid Receptor synthetic binding sites in GR18 cell line

PROVIDER: PRJEB26483 | ENA |

REPOSITORIES: ENA

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synSTARR-seq with Glucocorticoid Receptor synthetic binding sites in GR18 cell line

Project description:We performed STARR-seq with synthetic libraries (synSTARR-seq) in GR18 cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for GR), upon glucocorticoid (dexamethasone) or vehicle (ethanol) treatment. The synthetic libraries are variants of the glucocorticoid receptor binding sites (GBS). The "GBS half site" library contains 8 consecutive randomized nucleotides within the core binding sites, while the "Cgt/Sgk library" contains 5 consecutive randomized nucleotides on the flank of the GBS.

2018-10-29 | E-MTAB-6737 | biostudies-arrayexpress

RNA-seq in GR18 cell line to identify genes regulated by the Glucocorticoid Receptor

Project description:To identify the genes regulated by the Glucocorticoid Receptor (GR), we performed RNA-seq in GR18 cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for GR), upon glucocorticoid (dexamethasone) or vehicle (ethanol) treatment.

2018-10-29 | E-MTAB-6738 | biostudies-arrayexpress

RNA-seq in GR18 cell line to identify genes regulated by the Glucocorticoid Receptor (24h treatment)

2019-03-11 | E-MTAB-7745 | biostudies-arrayexpress

RNA-seq in GR18 cell line to identify genes regulated by the Glucocorticoid Receptor

Project description:RNA-seq in GR18 cell line to identify genes regulated by the Glucocorticoid Receptor

| PRJEB26484 | ENA

ChIP-seq for H3K27ac in the GR18 cell line treated for 1.5 h with 1 µM dexamethasone

Project description:We performed ChIP-seq in the GR18 cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for GR), upon glucocorticoid (dexamethasone)treatment for 90 minutes. Cells were cross-linked with 1% formaldehyde for 3 minutes.

2019-03-11 | E-MTAB-7747 | biostudies-arrayexpress

RNA-seq in GR18 cell line to identify genes regulated by the Glucocorticoid Receptor (24h treatment)

Project description:RNA-seq in GR18 cell line to identify genes regulated by the Glucocorticoid Receptor (24h treatment)

| PRJEB31557 | ENA

ATAC-seq in GR18 cell line comparing cells treated for 90 minutes with 1 micro molar dexamethasone with vehicle (ethanol) control-treated cells

Project description:We performed ATAC-seq in the GR18 cell line (derived from U2OS ATTC:HTB-96, stably transfected with an expression construct for GR), upon glucocorticoid (dexamethasone) or vehicle (ethanol) treatment for 90 minutes.

2019-03-11 | E-MTAB-7746 | biostudies-arrayexpress

Cryptochromes mediate rhythmic repression of the glucocorticoid receptor

Project description:The circadian transcriptional repressors cryptochromes 1 (Cry1) and 2 (Cry2) interact with the C-terminus of the glucocorticoid receptor (GR) and are required for transrepression in response to the synthetic GR ligand dexamethasone (Dex) in mouse embryonic fibroblasts. Dex induction of many genes was increased in Cry-deficient fibroblasts suggesting that cryptochromes oppose transactivation in addition to contributing to transrepression. In mice, genetic loss of Cry1 and/or Cry2 resulted in glucose intolerance and constitutively high levels of circulating corticosterone, suggesting reduced glucocorticoid suppression of the hypothalamic-pituitary-adrenal axis coupled with increased sensitivity to the hyperglycemic effects of glucocorticoid-mediated transactivation in the liver. Cry1 and Cry2 association with a GRE in the Pck1 promoter was stimulated by Dex, and Dex-induced transcription of pck1 was strikingly increased in Cry-deficient livers. Finally, cry1-/-;cry2-/- mice subjected to 8 weeks of chronic Dex treatment exhibited incomplete suppression of circulating corticosterone and greater glucose intolerance compared with wildtype littermates subjected to the same chronic treatment, consistent with enhanced transcriptional response to the synthetic glucocorticoid ligand. Total RNA was obtained from WT and Cry1/2 KO MEFs treated with Dexamethasone (1uM) or control EtOH for 16 hours.

2011-12-22 | E-GEOD-24469 | biostudies-arrayexpress

Cryptochromes mediate rhythmic repression of the glucocorticoid receptor

2011-12-22 | GSE24469 | GEO

Identification of glucocorticoid receptor (GR) binding sites in multiple myeloma

Project description:Purpose: The glucocorticoid receptor is widely expressed across mutliple tissues, and yet has very tissue specfic actions. This is most likely due to the tissue specific chormatin landscape which dictates GR binding. It identify GR tragets in myeloma as potential therapeutic targets, we have used ChIP-seq to identify myeloma specific GR binding sites. Methods: DNA-chromatin complexes were isolated from MM.1S (GR positive) or MM.1RL (GR negative) cells following a 2 hour treatment with either 1 micromolar dexamethasone or vehicle control. Complexes were immunoprecipitated with either a GR specific antibody or an IgG control and the DNA isolated for next generation sequencing. Results: We identified 8689 high confidence GR binding sites and further correlated those sites to specific genes which are regulated by glucocorticoids through integration with gene expression array data from our lab (submitted to GEO speparately). Conclusions: Overall, our results indentified nearly 400 genes under the direct transcriptional control of glucocorticoids in myeloma cells.

2015-12-22 | GSE63209 | GEO

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