Project description:The objective of this study was to determine the gene expression profile of rat hepatocytes grown on decellularized rat liver matrices as a function of time.
Project description:We used the nanopore Cas9 targeted sequencing (nCATS) strategy to specifically sequence 125 L1HS-containing loci in parallel and measure their DNA methylation levels using nanopore long-read sequencing. Each targeted locus is sequenced at high coverage (~45X) with unambiguously mapped reads spanning the entire L1 element, as well as its flanking sequences over several kilobases. The genome-wide profile of L1 methylation was also assessed by bs-ATLAS-seq in the same cell lines (E-MTAB-10895).
Project description:We report the application of next generation sequencing technology for high-throughput profiling of cytosine methylation in adult rat liver tissue. To investigate the role of intragenic methylation, we generated the first high resolution methylation map of rat liver tissue and compared it with the proteome of the same rat tissue.