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Transcriptional Response of Ovine Lung to Infection with Jaagsiekte Sheep Retrovirus

ABSTRACT: Ovine pulmonary adenocarcinoma (OPA) is a chronic respiratory disease of sheep caused by jaagsiekte sheep retrovirus (JSRV). JSRV infects respiratory epithelial cells and initiates tumor growth. OPA is an important economic and welfare issue for sheep farmers but is also considered to be a valuable animal model for human lung adenocarcinoma. In this study, whole transcriptome profiling was performed on lung samples derived from JSRV-infected and uninfected lambs using RNA-seq.

PROVIDER: PRJEB27638 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2683940_1.fastq.gz	Fastqsanger.gz
	ERR2683940_2.fastq.gz	Fastqsanger.gz
	ERR2683950_1.fastq.gz	Fastqsanger.gz
	ERR2683950_2.fastq.gz	Fastqsanger.gz
	ERR2683941_1.fastq.gz	Fastqsanger.gz

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Project description:The Bashbay sheep (Ovis aries), an indigenous breed of Xinjiang, China, has many excellent characteristics. However, hybrids of Argali sheep and Bashbay sheep are susceptible to Mycoplasma ovipneumoniae infection, the causative agent of mycoplasma ovipneumonia, a chronic respiratory disease that is harmful to the sheep industry. To date, knowledge regarding the mechanisms responsible for M. ovipneumoniae pathogenesis is in scant. Herein, we report the results of transcriptome profiling of lung tissues from Argali hybrid sheep experimentally infected with an M. ovipneumoniae strain at 4 and 14 days post-infection, in comparison to mock-infected animals (0 d). Transcriptome profiling was performed by deep RNA sequencing, using the Illumina platform. The analysis of differentially expressed genes was performed to determine concomitant gene-specific temporal patterns of mRNA expression in the lungs after M. ovipneumoniae infection. We found 156 differentially expressed genes (44 up-regulated, 112 down-regulated) when comparing transcriptomic data at 4 and 0 days post-infection, and 367 (35 up-regulated, 332 down-regulated) when comparing 14 versus 0 days post-infection. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the differentially expressed genes at 4 and 14 versus 0 days post-infection were enriched in 109 and 150 pathways, respectively, and the Primary immunodeficiency pathway was considered most closely related to MO infection (p < 0.01). Hyper-IgM syndrome was identified based on the B-cell immunodeficiency signaling pathway from differentially expressed genes related M. ovipneumoniae infection. Gene Ontology analysis showed that differentially expressed genes in different groups were enriched for 497 and 928 terms, where those most closely related to M. ovipneumoniae infection are ciliated motor damage (p < 0.01). These results could aid in understanding why the Argali hybrid sheep are susceptible to MO as well as how M. ovipneumoniae infection progresses in the lungs and may provide useful information regarding key regulatory pathways.

Dataset Information

Transcriptional Response of Ovine Lung to Infection with Jaagsiekte Sheep Retrovirus

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