Metagenomic study of diversity of microorganisms in the salt mine of Pakistan
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB28016 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Hypersaline regions are terrestrial analogs of the Earth's primitive ecosystem and extraterrestrial environment. The salt range in Pakistan is considered among a few of the ancient salt deposits in the subcontinent. Karak salt mine is situated at the Northwest end in Pakistan. Despite the fact that halophiles initiated the formation of terrestrial ecosystems, their products and identities remain hidden. Some preliminary studies limited to culture-dependent isolations have been reported. Characterizing the microbiome that spans over centuries of ecosystem development is crucial, given their role in shaping landscape succession and biogeochemical cycles. Here, we used metagenomics techniques to explore the microbial diversity of the Karak salt mine. We used 16S rRNA Illumina amplicon sequencing to characterize the halophilic communities entrapped in Karak mine. The results were interpreted using Illumina Basespace, QIIME, and Cytoscape. Cultures were isolated at 16-25% salinity. Metagenomics data was consistent with our preliminary culturing data, indicating remarkable species to strain-level diversity of unique halophiles. A total of 107,099 (brine) and 122,679 (salt) reads were obtained. 16S rRNA based sequencing revealed a microbiome with bacteria (66% brine and 72% salt) dominated by Bacteroidetes and Proteobacteria with a strikingly high abundance of Archaea (18% brine and 13% salt). Alpha diversity has higher values in salt than in the brine. The study of the halophiles in the Karak salt mine provides clues for species contributing to the maintenance of biogeochemical cycles of the ecosystem. This is the first report of a metagenomic study of any hypersaline region of Pakistan.
| S-EPMC7386132 | biostudies-literature
Project description:Ground water Arsenic (As) toxicity is a global problem and millions of people are exposed to elevated levels (more than WHO advised maximum limit of 10µg/L) through drinking water. The exposure is associated with various cancerous and non-cancerous diseases. It may alter DNA methylation profiles of inviduals and suppress the activity of various genes giving rise to different diseases. Pakistan, a developing country in South Asian region, also has reported elevated ground water As levels in various investigations since 2005. However, a very limited biomonitoring studies have been conducted in this context while no study reports molecular changes associated with drinking water As exposure in Pakistan. Within this context, the present study aimed to investigate genome-wide DNA methylation profiles of the exposed subjects in two districts of Punjab Province Pakistan, i.e Lahore and Kasur. The population was stratified into three exposure groups comprising Low, Medium and High exposure based on their urinary arsenic levels. Genome-wide DNA methylation profiles were obtained using MeDIP in combination with NimbleGen 2.1M Deluxe Promotor arrays.
2021-10-27 | GSE157111 | GEO
Project description:Ground water Arsenic toxicity is the global problem and millions of people are exposed to elevated levels through drinking water than WHO permiscible limit of 10µg/L. The exposure is associated with various cancerous and non-cancerous diseases. It may alter the gene expression profile of invidual and suppress the activity of various genes giving rise to different diseases. Pakistan, a developing country in South Asian region, also have different areas reported to have elevated Ground water As. levels since 2005. The Present Study aimed to investigate Transcriptome profile of the exposed subjects in two districts of Punjab Province Pakistan. i.e Lahore and Kasur. The population was stratified into three exposure groups comprising Low, Medium and High exposure based on their urinary arsenic levels. We used Agilent Microarrays platform for Transcriptomics analysis and Linear mixed model was used to find differential gene expression associated with Arsenic exposure
2019-12-26 | GSE110852 | GEO
Project description:Clinical isolate from Pakistan
| PRJNA33789 | ENA
Project description:Effect of certain microorganisms on mouse gut microbial communities in aged group.
2021-09-24 | GSE180087 | GEO
Project description:Investigation of global gene expression changes in Saccharomyces cerevisiae strain NRRL Y-12632 (ATCC® 18824) grown in media made with asbestos mine tailings-laden water compared to the control grown in media made with double distilled water
2017-02-24 | GSE89875 | GEO
Project description:The increasing global human population has been associated with the development of high-density urban communities. This has led to increase in fossil energy consumption and posed serious threats to the environment and human health. Organosulfur compounds found in crude oil and transportation fuels such as diesel have gained strong attention because they are hazardous to human and the ecosystem. Moreover, the sulfur oxide gases resulting from fuel combustion are a major cause of acid rain. Governments and environmental organizations worldwide have recognized the problem and implemented strict regulations and legislations that limit the amount of sulfur in diesel. Hydrodesulphurization (HDS) is commonly used by oil refineries to reduce sulfur content in refined fuels. However, HDS has many disadvantages. It is costly, environmentally polluting, and not sufficiently efficient. Accordingly, there has been increasing interest in the development of alternative desulfurization technologies to circumvent the problems associated with the conventional HDS. Biodesulfurization (BDS) has emerged as an alternative or a complement technology to overcome the drawbacks of the conventional HDS. BDS exploits the ability of dedicated microorganisms to remove sulfur from many organosulfur compounds that are commonly found in crude oil and refined fuels. As compared to thermochemical treatments like HDS, BDS is environmentally friendly, cost-effective and active towards organosulfur compounds that escape the conventional HDS. Nonetheless, lack of deep understanding of the physiology and metabolism, particularly sulfur metabolism, of biodesulfurizing microbes has impeded the development and implementation of a commercially viable BDS process. In this project, we apply metabolomics and proteomics to better understand the physiological adaptations and sulfur metabolism of in a model biodesulfurization-competent strain Rhodococcus qingshengii IGTS8.
2021-08-09 | PXD021362 | Pride
Project description:Metagenomic Shotgun of EPS-cultivated microorganisms
| PRJEB24069 | ENA
Project description:The pyrite mine of Lousal, Grândola, Portugal, was discovered in 1882 and extensively exploited from 1900 until its closure in 1988 when the mining ores were not viable economically. Aiming at becoming a cultural-touristic center, the mining area was rehabilitated raising a Mining Museum and a Science Centre along with the reopening of a mining gallery for public access. In recent times, aerobiological studies on subterranean environments such as caves, mines and archeological necropolis revealed to be an important source of novel bacteria, from which their biotechnological potential are under study. Here we present a Pseudomonas sp. isolated from the mine air with potential secondary metabolite biosynthesis, resistance to antibiotics and virulence factors.
2021-11-05 | GSE186946 | GEO