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Chip-seq of BlaER1 B cell transdifferentiation

ABSTRACT: Chip-seq of BlaER1 B cell transdifferentiation

PROVIDER: PRJEB28056 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2722690.fastq.gz	Fastqsanger.gz
	ERR2722691.fastq.gz	Fastqsanger.gz
	ERR2722692.fastq.gz	Fastqsanger.gz
	ERR2722693.fastq.gz	Fastqsanger.gz

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Comparative transcriptomics of B cell transdifferentiation in human and mouse. ChIP-Seq data

Project description:Exogenous overexpression of CEBPA transcription factor induces transdifferentiation from pro B cells into functional macrophages. Here we report the CEBPA binding ChIP-Seq data at 12 hours time point after induction of transdifferentiation in human BLaER1 cells (Rapino et al. 2013).

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Epigenetic analysis of BlaER1 transdifferentiation

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Project description:BLaER1 is a human B cell precursor leukemia cell line derived from the RCH-ACV cells. These cells are stably infected with a construct that overexpresses the transcription factor C/EBPa fused with the estrogen receptor hormone binding domain (ER) and GFP. Upon induction with beta-estradiol, C/EBP is internalized into the nucleus, promoting massive transcriptional changes and inducing the transdifferentiation of these pre-B cells into functional macrophages. This process, that lasts 7 days, can be monitored by the detection of specific B cell and macrophage surface markers by flow cytometry. With the goal of understanding the interplay between chromatin and transcription, we have generated transcriptomic data by RNA-Seq in twelve time points along the transdifferentiation process, in two biological replicates.

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