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Population Genetics Predicts Hotspot Dosages for Antibiotic Resistance Evolution

ABSTRACT: Recreation of evolutionary dynamics of Escherichia coli growing in the presence of different erythromycin concentrations during 5 days.

PROVIDER: PRJEB28068 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2730420_1.fastq.gz	Fastqsanger.gz
	ERR2730420_2.fastq.gz	Fastqsanger.gz
	ERR2730430_1.fastq.gz	Fastqsanger.gz
	ERR2730430_2.fastq.gz	Fastqsanger.gz
	ERR2730440_1.fastq.gz	Fastqsanger.gz

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Project description:In this study we have used the rifampicin selection as a tool to genetically improve the erythromycin producer Saccharopolyspora erythraea. Two rifampicin-resistant (rif) mutants, rif1 and rif6, have been characterized in more detail. With respect to the parental strain NRRL2338, rif1 (harboring the missense S444F) exhibited higher respiratory performance and final erythromycin yields; in contrast, rif6 (harboring the missense Q426R) was slow-growing, developmental-defective and severely impaired in erythromycin production. The results of genome-wide analysis of expression profiles using DNA micro-arrays demonstrated that these mutations deeply changed the transcriptional profile of S. erythraea with marked regional distribution. Keywords: mutants versus wild type comparison in a time course experiment

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