Ontology highlight
ABSTRACT:
ORGANISM(S): Saccharopolyspora erythraea
SUBMITTER: Giorgio Corti
PROVIDER: E-GEOD-12017 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Carata Elisabetta E Peano Clelia C Tredici Salvatore M SM Ferrari Francesco F Talà Adelfia A Corti Giorgio G Bicciato Silvio S De Bellis Gianluca G Alifano Pietro P
Microbial cell factories 20090330
<h4>Background</h4>There is evidence from previous works that bacterial secondary metabolism may be stimulated by genetic manipulation of RNA polymerase (RNAP). In this study we have used rifampicin selection as a strategy to genetically improve the erythromycin producer Saccharopolyspora erythraea.<h4>Results</h4>Spontaneous rifampicin-resistant (rif) mutants were isolated from the parental strain NRRL2338 and two rif mutations mapping within rpoB, S444F and Q426R, were characterized. With resp ...[more]