Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Phenotype and expression profile of S. erythraea rifampicin-resistant (rif) mutants affected in erythromycin production


ABSTRACT: In this study we have used the rifampicin selection as a tool to genetically improve the erythromycin producer Saccharopolyspora erythraea. Two rifampicin-resistant (rif) mutants, rif1 and rif6, have been characterized in more detail. With respect to the parental strain NRRL2338, rif1 (harboring the missense S444F) exhibited higher respiratory performance and final erythromycin yields; in contrast, rif6 (harboring the missense Q426R) was slow-growing, developmental-defective and severely impaired in erythromycin production. The results of genome-wide analysis of expression profiles using DNA micro-arrays demonstrated that these mutations deeply changed the transcriptional profile of S. erythraea with marked regional distribution. Keywords: mutants versus wild type comparison in a time course experiment Total of 12 Samples

ORGANISM(S): Saccharopolyspora erythraea

SUBMITTER: Giorgio Corti 

PROVIDER: E-GEOD-12017 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Phenotypes and gene expression profiles of Saccharopolyspora erythraea rifampicin-resistant (rif) mutants affected in erythromycin production.

Carata Elisabetta E   Peano Clelia C   Tredici Salvatore M SM   Ferrari Francesco F   Talà Adelfia A   Corti Giorgio G   Bicciato Silvio S   De Bellis Gianluca G   Alifano Pietro P  

Microbial cell factories 20090330


<h4>Background</h4>There is evidence from previous works that bacterial secondary metabolism may be stimulated by genetic manipulation of RNA polymerase (RNAP). In this study we have used rifampicin selection as a strategy to genetically improve the erythromycin producer Saccharopolyspora erythraea.<h4>Results</h4>Spontaneous rifampicin-resistant (rif) mutants were isolated from the parental strain NRRL2338 and two rif mutations mapping within rpoB, S444F and Q426R, were characterized. With resp  ...[more]

Similar Datasets

2009-04-12 | GSE12017 | GEO
2012-05-23 | E-GEOD-30600 | biostudies-arrayexpress
2016-06-22 | GSE65007 | GEO
2016-06-22 | E-GEOD-65007 | biostudies-arrayexpress
2020-02-26 | GSE134767 | GEO
2006-03-08 | GSE4395 | GEO
2006-03-08 | E-GEOD-4395 | biostudies-arrayexpress
| PRJNA113291 | ENA
2020-08-04 | GSE144067 | GEO
2022-05-21 | GSE150974 | GEO