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RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with BC094916 against vector control

ABSTRACT: RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with BC094916 against vector control

PROVIDER: PRJEB28132 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2730830_1.fastq.gz	Fastqsanger.gz
	ERR2730830_2.fastq.gz	Fastqsanger.gz
	ERR2730829_1.fastq.gz	Fastqsanger.gz
	ERR2730829_2.fastq.gz	Fastqsanger.gz

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RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with Gm40600 against vector control

Project description:We found that some novel genes such as Gm40600 mRNA significantly changed in plasma cells. Because of plasmablast-like, mus musculus myeloma SP 2/0 cell line was selected to test the effect of Gm40600 overexpression on plasmablast/plasma cells. Gm40600 cDNA was cloned into lentiviral vector LV201 to generate Gm40600 protein. Gm40600-expressing LV201 was then transfected into SP 2/0 cells, and stable transfectants were identified by drug selection. To identify the effect of Gm40600 overexpression on gene expression, we determined mRNA profiles in Gm40600-overexpressed and vector-transduced SP 2/0 cells by RNA-sequencing. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.

2019-07-15 | E-MTAB-7136 | biostudies-arrayexpress

RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with Gm614 against vector control

Project description:We found that a novel gene Gm614 mRNA significantly changed in plasma cells. Because of plasmablast-like, Mus musculus myeloma SP 2/0 cell line was selected to test the effect of Gm614 overexpression on plasmablast/plasma cells. Gm614 cDNA (General Biosystems, Anhui, China) was cloned into lentiviral vector LV201 (Fugene Corp., Guangzhou, China) to generate Gm614 protein. Gm614-expressing LV201 was then transfected into SP 2/0 cells, and stable transfectants were identified by drug selection (Puromycin, Sigma, 10 μg/ml). To identify the effect of Gm614 overexpression on gene expression, we determined mRNA profiles in Gm614-overexpressed and vector-transduced SP 2/0 cells by RNA-seq. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.

2020-08-10 | E-MTAB-7135 | biostudies-arrayexpress

RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with Gm40600 against vector control

Project description:RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with Gm40600 against vector control

| PRJEB28189 | ENA

RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with Gm614 against vector control

Project description:RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with Gm614 against vector control

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RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with BC094916 against vector control

Project description:We found that a novel gene BC094916 mRNA significantly decreased in plasma cells. Because of plasmablast-like, mus musculus myeloma SP 2/0 cell line was selected to test the effect of BC094916 overexpression on plasmablast/plasma cells. BC094916 cDNA (General Biosystems, Anhui, China) was cloned into lentiviral vector LV122 or LV201 (Fugene Corp., Guangzhou, China) to generate BC094916 and BC094916-EGFP fusion protein, respectively. BC094916-expressing LV122 was then transfected into SP 2/0 cells, and stable transfectants were identified by drug selection (Puromycin, Sigma, 10 μg/ml). BC094916 overexpression suppressed SP 2/0 cell proliferation by inducing cell apoptosis. Importantly, BC094916 overexpression effectively suppressed tumor progression in the SP 2/0 xenograft mouse model. In addition, we found that BC094916 is a suppressive transcriptional factor. To verify its target genes, we determined mRNA profiles in BC094916-overexpressed and vector-transduced SP 2/0 cells by RNA-seq. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.

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RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with Gm6377 or Loc108167440 against vector control

Project description:We found that novel gene Gm6377 or Loc108167440 mRNA significantly changed in plasma cells. Because of plasmablast-like, mus musculus myeloma SP 2/0 cell line was selected to test the effect of Gm6377 or Loc108167440 overexpression on plasmablast/plasma cells. Gm6377 or Loc108167440 cDNA was cloned into lentiviral vector LV201. Gm6377 or Loc108167440-expressing LV201 was then transfected into SP 2/0 cells, and stable transfectants were identified by drug selection. To identify the effect of Gm6377 or Loc108167440 overexpression on gene expression, we determined mRNA profiles in Gm6377 or Loc108167440-overexpressed and vector-transduced SP 2/0 cells by RNA-sequencing. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.

2020-08-13 | E-MTAB-7139 | biostudies-arrayexpress

RNA-seq of mouse myeloma cell line SP 2/0 cells overexpressed with LOC108168067 against vector control

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2019-07-31 | E-MTAB-7111 | biostudies-arrayexpress

RNA-seq of mouse myeloma cell line SP 2/0 cells overexpressed with LOC108168067 against vector control

Project description:RNA-seq of mouse myeloma cell line SP 2/0 cells overexpressed with LOC108168067 against vector control

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RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with Gm6377 or Loc108167440 against vector control

Project description:RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with Gm6377 or Loc108167440 against vector control

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Transcription profiling of human multiple myeloma cells to compare the effect of CS1 expression levels.

Project description:We used microarrays to detail the global programme of gene expression underlying CS1-regulated biological processes including increased cell adhesion and cell proliferation. Experiment Overall Design: Human multiple myeloma cell lines with and without CS1 expression, either by transffecting CS1 overexpressing vector or lentiviral CS1 shRNA, were subject to total RNA extraction and hybridization on Affymetrix microarrays. We sought to define the common genes underexpressed in CS1 knockdown myeloma cells whereas overexpressed in CS1 overexpressing myeloma cells to gain insight into CS1-targeting genes in order to define the molecular mechanisms regulated by CS1 in multiple myeloma.

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