Project description:Species identification of fragmentary bones remains a challenging task in archeology and forensics. A species identification method for such fragmentary bones that has recently attracted interest is the use of bone collagen proteins. We developed a method similar to DNA barcoding that reads collagen protein sequences in bone and automatically determines the species by performing sequence database searches. We tested our method using bone samples from 30 vertebrate species ranging from mammals to fish.
Project description:To address the delivery barriers of macromolecular therapeutics, we sought to develop high-throughput peptide barcoding platform as a homogeneous method for macromolecular screening. Implemented in open-source algorithms, BarcodeBabel and PeptideBabel, this approach permits the design of libraries of peptide barcodes and novel penetration sequences optimized for analysis by quantitative mass spectrometry.
Project description:To investigate immunoediting at the primary tumour, we used DNA barcoding combined with NGS. By stably integrating 4T1 murine cancer cell line with 250000 unique DNA barcodes (1 barcode per cell), we can trace how barcode (and therefore subclonal) diversity changes over time and in the presence of the endogenous immune system, compared to immunocompromised mice.
Project description:We describe the development of a high-sensitivity protein quantification system called HaloTag protein barcoding assay. The assay involves target protein linking to a unique molecule-counting oligonucleotide by click chemistry.
