Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Small RNA seq of Drosophila ovaries expressing FHVdlB2eGFP replicon

PROVIDER: PRJEB28601 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2790950.fastq.gz	Fastqsanger.gz
	ERR2790951.fastq.gz	Fastqsanger.gz
	ERR2790952.fastq.gz	Fastqsanger.gz
	ERR2790953.fastq.gz	Fastqsanger.gz
	ERR2790954.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

Impact of depeltion of CG5694/ova on transcription in Drosophila ovaries [miRNA- and RNA-seq]

Project description:Purpose: To analysis Ova impact on transcription in Drosophila ovaries by mRNA-seq and small RNA-seq Methods: mRNA-seq and small RNA-seq of RNA extracted from Drosophila ovaries Results: Ova has no obvious impact on protein coding genes expression or piRNA biogenesis.

2019-01-14 | GSE104924 | GEO

Project description:small RNA-seq and mRNA-seq of Drosophila Belle transgenic ovaries

| PRJNA494685 | ENA

Drosophila melanogaster strain:Oregon RI

Project description:Temperature shapes small RNA profiles in Drosophila ovaries

| PRJNA312441 | ENA

mRNA expression in iPS cells generated by a synthetic self-replicative RNA

Project description:We generated iPS cells with a synthetic self-replicative RNA that expresses four independent reprogramming factors (OCT4, KLF4, SOX2 and either c-MYC or GLIS1). We performed whole genome RNA sequencing (RNA-seq) of iPS cell clones, parental BJ and HUES9 ES cell controls. All iPS cell clones analyzed by RNA-seq showed unsupervised hierarchical clustering and expression signatures characteristic of human HUES9 ES cells that were highly divergent from parental human fibroblasts. RNA-seq in two OKS-iM iPS clones (generated from OCT4, KLF4, SOX2 and cMYC expressing RNA replicon), two OKS-iG clones (generated from OCT4, KLF4, SOX2 and GLIS1 expressing RNA replicon), HUES9 and BJ cells.

2014-04-25 | E-GEOD-38265 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Drosophila melanogaster small RNAs in ovaries

| PRJNA215819 | ENA

H2A.Z regulates zygotic genome activation

Project description:Affinity Purification Mass Spectrometry (AP-MS) of Drosophila ovaries expressing an H2A.Z-FlagHA transgene to identify interacting partners of H2A.Z to elucidate potential maternally supplied histone chaperones that deposit H2A.Z on the transcription start site (TSS).

2022-02-15 | PXD029061 | Pride

Next-generation sequencing analysis of Piwi-interacting RNAs (piRNAs) in Drosophila wild-type and bel mutant ovaries

Project description:Belle has been known to be co-localized with piRNA-related proteins at the nuage of germline cells during Drosophila oogenesis. However, its role in piRNA biogenesis remains unclear. To reveal whether Belle is involved in regulating piRNA expression, we performed next-generation sequencing analysis of small non-coding RNAs on ovaries harvested from the wild type (W1118) and trans-heterozygous bel[74407/neo30] mutant. Small RNA-seq experiments were performed on three individual ovary samples with the same genotype. For piRNA expression analysis, we performed mapping of three sets of small RNA sequencing reads for each genotype to previously identified eight distinct piRNA clusters located in four different Drosophila chromosomes (from X to 4). Analysis of the piRNA expression profiling from these piRNA cluster loci indicates that some specific piRNA populations were either upregulated or downregulated in bel mutant ovaries compared with wild-type ovaries. Furthermore, we performed systematic analysis by mapping piRNA sequencing reads to sequences of all identified Drosophila transposable elements (TEs) to classify and measure piRNA reads based on their TE targets. Among 124 TE-classified piRNA populations, 9 and 20 of them were upregulated and downregulated (≥2 folds), respectively, in bel74407/neo30 mutant ovaries compared with those from wild-type ovaries. To examine the effect of the bel[74407/neo30] mutation on the ping-pong cycle for secondary piRNA biogenesis, analysis of the ping-pong signature of piRNAs specifically mapped to the retro-element Burdock was performed. The ping-pong signature for the generation of secondary piRNAs was not significantly altered in bel mutants compared with the wild type. These results, taken together, indicate that Bel is not required for primary and secondary piRNA biogenesis, but it is involved in regulating expression of specific subsets of piRNA populations.

2017-09-01 | GSE65565 | GEO

Project description:Small RNA sequencing of mutant Huntingtin expressing Drosophila

| PRJNA658796 | ENA

Impact of CG5694/ova on H3K4me2 and pol II patterns in Drosophila ovaries [ChIP-seq]

Project description:Purpose: To analysis Ova impact on H3K4me2 and pol II patterns in Drosophila ovaries by ChIP-seq Methods: Chromatin Immunoprecipitation to identify H3K4me2 and pol II patterns in Drosophila ovaries Results: Knockdown of Ova increases of H3K4me2 and Pol II in some genomic regions.

2019-01-14 | GSE104923 | GEO

gammaH2Av ChIP-seq in Drosophila stage 10B and 13 follicle cells

Project description:We analyzed gamaH2Av ChIP-seq from hand dissected stage 10B and 13 follicle cell nuclei. Egg chambers were dissected from wild-type (OrR) or H2Av[ΔCT] ovaries to assess binding at the Drosophila Follicle Cell Amplicons and across the genome. ChIP-seq of gammaH2Av bound to follicle cell DNA from stage 10B and 13 egg chambers, collected from wild-type (OrR) and H2Av[ΔCT] Drosophila ovaries. Sequences analyzed by Illumina sequencing. Two replicates are included for each ChIP reaction.

2015-06-18 | E-GEOD-66689 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data