Project description:Comparative genome hybridization (CGH) of sample of 58 Bordetella bronchiseptica, B. pertussis, and B. parapertussis strains selected to span four clusters as defined by MLST.
Project description:Comparative genomic hybridization (CGH) of 71 Bordetella parapertussis strains isolated from either humans or sheep, and 1 B. bronchiseptica strain isolated from a rabbit
Project description:To our knowledge, we provide the first proteomics study of Bordetella parapertussis, one of the causative agents of whooping cough. We compared the identified proteins different to Bordetella pertussis, the other pathogen causing whooping cough. In addition, we extended the study to investigate the proteome response to iron limitation, a stress condition the pathogens face while infection.
Project description:The classical bordetellae (Bordetella pertussis, B. parapertussis, and B. bronchiseptica) are obligate aerobes that use only oxygen as their terminal electron acceptor for electron transport-coupled oxidative phosphorylation. Therefore, access to oxygen is critical for these bacteria to survive. To better understand how B. bronchiseptica changes its gene regulation when faced with different levels of oxygen, we grew liquid cultures of B. bronchiseptica RB50 in ambient air, 5% oxygen, and 2% oxygen. We also measured how the presence of 5% carbon dioxide affected gene expression in these bacteria, since they are respiratory pathogens and therefore get exposed to higher carbon dioxide levels during infection than are found in ambient air.