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Transcriptomic responses of two population strains of the kelp Saccharina latissima to temperature and salinity changes

ABSTRACT: Transcriptomic responses of two population strains of the kelp Saccharina latissima to temperature and salinity changes

PROVIDER: PRJEB29430 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2861930_1.fastq.gz	Fastqsanger.gz
	ERR2861930_2.fastq.gz	Fastqsanger.gz
	ERR2861940_1.fastq.gz	Fastqsanger.gz
	ERR2861940_2.fastq.gz	Fastqsanger.gz
	ERR2861950_1.fastq.gz	Fastqsanger.gz

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Transcriptomic responses of two population strains of the kelp Saccharina latissima to temperature and salinity changes

Project description:The data is a result of a large laboratory experiment targeting interactive effects of salinity and temperature on the transcriptomic level in algae from two populations of an ecologically relevant kelp species (Laminariales), Saccharina latissima. Research interest in S. latissima has recently been increasing given its importance as ecosystem engineer along temperate rocky shores in the Atlantic Ocean, and its growing potential in industrial applications such as aquaculture, pharmaceutics, food and feed. Young sporophytes of S. latissima were raised from stock cultures of clonal male and female gametophytes at the Alfred-Wegener-Institute Helmholtz Centre for Polar and Marine Research. Cultures originated from sporophyte collected at Kongsfjorden (79°N, 11°E; Spitsbergen, Norway) and at Roscoff (48° 43′ 39″ N, 3° 59′ 13.2″ W; Brittany, France). Sporophytes of both populations were grown aerated in glass beakers at 8°C and under a photon fluence rate of 20 µmol photons m–2 s–1 of photosynthetically active radiation with a 18 h light: 6 h dark photoperiod and were cultivated in sterile seawater enriched with Provasoli (Starr & Zeikus 1993) with an absolute salinity (SA) of ~30 during three months. At the start of the experiment, sporophytes were either kept at 8°C (or transferred to 0°C and 15°C) in temperature controlled rooms. After one week, per each temperature, sporophytes were divided into a low salinity treatment of SA 20 or kept at the control salinity (SA 30).

2019-07-08 | E-MTAB-7348 | biostudies-arrayexpress

Temperature dependent sex-biased gene expression in the gametophytes of the kelp Saccharina latissima

Project description:We performed a laboratory experiment with vegetative gametophytes of the kelp Saccharina latissima and exposed the gametophytes to three temperatures (4°C, 12°C and 20°C) by sex (female, male) for 14 days.

2019-09-25 | E-MTAB-8267 | biostudies-arrayexpress

Light and temperature induced gene expression in Saccharina latissima

Project description:Effect of PAR and temperature stress on the gene expression Saccharina latissima. Total RNA of stress treatments (low PAR 2° and 17°C, high PAR 2° and 17°C) was hybridized against the control treatment (low PAR 12°C); hybridizations were carried out in 4 replicates.

2012-01-07 | E-MEXP-3450 | biostudies-arrayexpress

Saccharina latissima

Project description:Saccharina latissima overview

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Saccharina latissima

Project description:transcriptome of Saccharina latissima

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Saccharina latissima gametogenesis

Project description:Male and female gametogenesis in the kelp Saccharina latissima (Laminariales, Laminariaceae)

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Saccharina latissima microbiota

Project description:Exploring the Saccharina latissima microbiota

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Temperature dependent sex-biased gene expression in the gametophytes of the kelp Saccharina latissima

Project description:Temperature dependent sex-biased gene expression in the gametophytes of the kelp Saccharina latissima

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Single-base methylome profiling of the giant kelp Saccharina japonica reveals significant differences in DNA methylation to plants and animals

Project description:Kelp are the largest photosynthetic organisms in the ocean with tissue differentiation and complex life cycles. Other multicellular organisms with similar complexity such as plants and animals are well known to posses epigenetic mechanisms such as DNA methylation to control development and morphogenesis. Despite plant-like body plans and the presence of different life-cycle stages, the kelp species Saccharina japonica has only a very low level of DNA methylation, yet we have found strong evidence for differential methylation of regulatory elements and protein-coding genes which seem to contribute to the formation of life-cycle stages, tissue differentiation, growth and halogen metabolism. Thus, DNA methylation seems to play an important role in kelp, which has not been reported before.

2018-07-17 | GSE117191 | GEO

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