Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Whole genome sequence-based surveillance of Mycobacterium tuberculosis strains in Portugal

PROVIDER: PRJEB29446 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR10219862_1.fastq.gz	Fastqsanger.gz
	ERR10219862_2.fastq.gz	Fastqsanger.gz
	ERR10219863_1.fastq.gz	Fastqsanger.gz
	ERR10219863_2.fastq.gz	Fastqsanger.gz
	ERR10219864_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 2332

Similar Datasets

Project description:Whole genome sequence-based surveillance of M. tuberculosis strains in Portugal

| PRJEB29447 | ENA

Project description:Whole genome sequence-based surveillance of Legionella pneumophila strains in Portugal

| PRJEB65289 | ENA

Project description:Whole genome sequence-based surveillance of Neisseria meningitidis strains in Portugal

| PRJEB36474 | ENA

Project description:Whole genome sequence-based surveillance of S. pneumoniae strains in Portugal

| PRJEB35114 | ENA

Project description:Whole genome sequence-based surveillance of Clostridium difficile strains in Portugal

| PRJEB49792 | ENA

Project description:Whole genome sequence-based surveillance of Haemophilus influenzae strains in Portugal

| PRJEB26586 | ENA

Project description:Whole genome sequence-based surveillance of Escherichia coli strains in Portugal

| PRJEB54735 | ENA

Project description:Whole genome sequence-based surveillance of Nontuberculous Mycobacteria strains in Portugal

| PRJEB57933 | ENA

Project description:Whole genome sequence-based surveillance of Salmonella enterica strains in Portugal

| PRJEB32515 | ENA

A safe alternative to H37Rv: the attenuated double auxotroph Mycobacterium tuberculosis ΔleuDΔpanCD

Project description:Currently available model organisms such as Mycobacterium smegmatis and Mycobacterium bovis Bacillus Calmette-Guérin (BCG) have significantly contributed to our understanding of tuberculosis (TB) biology, these models have limitations such as differences in genome size, growth rates and virulence. Attenuated Mycobacterium tuberculosis strains may provide more representative, safer models to study M. tuberculosis biology. For example, the M. tuberculosis ΔleuDΔpanCD double auxotroph, has undergone rigorous in vitro and in vivo safety testing. Like other auxotrophic strains, this has subsequently been approved for use in biosafety level (BSL) 2 facilities. Auxotrophic strains have been assessed as models for drug-resistant M. tuberculosis and for studying latent TB. These offer the potential as safe and useful models, but it is important to understand how well these recapitulate salient features of non-attenuated M. tuberculosis. We therefore performed a comprehensive comparison of M. tuberculosis H37Rv and M. tuberculosis ΔleuDΔpanCD. These strains demonstrated similar in vitro and intra-macrophage replication rates, similar responses to anti-TB agents and whole genome sequence conservation. Shotgun proteomics analysis suggested that M. tuberculosis ΔleuDΔpanCD has an increased propensity to enter a dormant state during acid stress, which has been verified using a dual-fluorescent replication reporter assay. Importantly, infection of human peripheral blood mononuclear cells with the 2 strains elicited comparable cytokine production, demonstrating the suitability of M. tuberculosis ΔleuDΔpanCD for immunological assays. We provide comprehensive evidence to support the judicious use of M. tuberculosis ΔleuDΔpanCD as a safe and suitable model organism for M. tuberculosis research, without the need for a BSL3 facility.

2019-07-30 | PXD013677 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data