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ABSTRACT: Whole genome sequence-based surveillance of Legionella pneumophila strains in Portugal

PROVIDER: PRJEB65289 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	ERR11862821_1.fastq.gz	Fastqsanger.gz
	ERR11862821_2.fastq.gz	Fastqsanger.gz
	ERR11862822_1.fastq.gz	Fastqsanger.gz
	ERR11862822_2.fastq.gz	Fastqsanger.gz
	ERR11862823_1.fastq.gz	Fastqsanger.gz

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Legionella ITS oligonucleotide microarray

Project description:In this report, we have developed a rapid oligonucleotide microarray detection technique to identify the most common ten Legionella spp.. The sensitivity of the detection was at 1.0 ng with genomic DNA or 13 CFU/100 mL with Legionella cultures. The microarray detected seven air conditioner-condensed water samples with 100% accuracy, validating the technique as a promising method for applications in basic microbiology, clinical diagnosis, food safety, and epidemiological surveillance. The phylogenetic study based on the ITS has also revealed interestingly that the non-pathogenic L. fairfieldensis is the closest to L. pneumophila than the nine other pathogenic Legionella spp..

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