Project description:The six species of lungfish possess both lungs and gills and are the closest extant relatives of tetrapods. Here, we report a single-cell transcriptome atlas of the West African lungfish (Protopterus annectens). This species manifests the most extreme form of terrestrialization, a life history strategy to survive dry periods in five lungfish species that can last for years, characterized by dormancy and reversible adaptive changes of the gills and lungs. Our atlas highlights the cell type diversity of the West African lungfish, including gene expression consistent with phenotype changes of terrestrialization. Comparisons with terrestrial tetrapods and ray-finned fishes revealed broad homology between the swim bladder and lung cell types as well as shared and idiosyncratic changes of the external gills of the West African lungfish and the internal gills of Atlantic salmon. The single-cell atlas presented here provides a valuable resource for further exploring the evolution of the vertebrate respiratory system and the diversity of lungfish terrestrialization.
Project description:Transcripts from the human and mouse CAPN10 cDNA sequences cloned into pcDNA and expressed in cultured cells undergo unexpected splicing. Peptide sequences missing from the spliced version of CAPN10 expressed in COS7 cells were determined by MS analysis.
Project description:We report the presence of microRNA in the supraspliceosome in six different HeLa samples. After the extraction of the supraspliceosome we subjected its small RNA content to NGS. We identified 304 sequences derived from pre-miRNAs. About two thirds of them are mature miRNAs, which likely shuttled back from the cytoplasm, whereas the rest account for various defined regions of the pre-miRNA. Out of these 304 pre-miRNA-derived sequences, 95 were undetected in cellular extract, and the abundance of additional 58 strongly differed from that in cellular extract.
