Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

VRE isolates from entry-screening isolated in Germany

ABSTRACT: VRE isolates from entry-screening isolated in Germany

PROVIDER: PRJEB29744 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2930910_1.fastq.gz	Fastqsanger.gz
	ERR2930910_2.fastq.gz	Fastqsanger.gz
	ERR2930920_1.fastq.gz	Fastqsanger.gz
	ERR2930920_2.fastq.gz	Fastqsanger.gz
	ERR2930930_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 190

Similar Datasets

Haemophilus isolates from Germany

Project description:Haemophilus isolates from Germany

| PRJEB43356 | ENA

Metagenomic screening isolates

Project description:Metagenomic screening isolates Raw sequence reads

| PRJNA477909 | ENA

Study of clinical VRE

Project description:Study of clinical VRE

| PRJEB13267 | ENA

Enterococcus faecium strain:VRE

Project description:Enterococcus faecium strain:VRE Genome sequencing

| PRJNA589567 | ENA

VRE ST796 in Switzerland

Project description:Sequencing of VRE in Switzerland, 2018

| PRJEB27159 | ENA

Differential Whole Genome CRISPR Screening Strategy for SARS-CoV-2 Spike and VSV-G Mediated Pseudoviral Entry

Project description:The coronavirus disease 2019 (COVID-19) has caused over 6 million deaths worldwide and disrupted the global economy. The causative agent for this disease, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes mild to lethal respiratory infections. Understanding the cellular host factors that promote and inhibit for SARS-CoV-2 infection is important for identifying virial countermeasures. Moreover, new methods are needed to be to identify host genes influencing specific steps of viral infections. Here, we developed a CRISPR whole genome screen against SARS-CoV-2 spike enveloped retro-pseudoviruses with a GFP reporter to specifically identify host genes that facilitate viral entry. By including two counter screen strategies, this approach can be used to distinguish host genes affecting the pseudoviral reporter from those unique to envelope-mediated entry. First, an alternate envelope, VSV-G allowed identification of shared genes associated with retro-transcription, integration and reporter expression. Second, a recently developed Cre-Gag fusion pseudovirus bypassed retro transcription and integration by directly activating a floxed GFP reporter. Our approach correctly identified SARS-CoV-2 and VSV-G receptors ACE2 and LDLR, respectively and distinguished genes associated with retroviral reporter expression from envelope mediated entry. Overall, this work provides a new strategy for screening genes influencing envelope mediated entry without the complexity of live-viral screens which are complicated with large numbers of genes associated with all aspects of viral pathogenesis and replication. This approach should be of use for identifying genes contributing to and inhibiting SARS-CoV-2 entry and provide a platform for the analysis of newly emerging viruses.

2022-06-27 | GSE206996 | GEO

VRE genomes Australia

Project description:Genomic analyses of VRE at an Australian public hospital

| PRJEB25449 | ENA

VRE hospital epidemiology Germany

Project description:Whole genome sequencing of 773 VREfm patient samples

| PRJEB30772 | ENA

VRE hospital epidemiology Germany

Project description:Whole genome sequencing of 773 VREfm patient samples

| PRJEB30783 | ENA

Enterococcus faecium strain:VRE-3

Project description:Enterococcus faecium strain:VRE-3 Genome sequencing