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Blumeria graminis f.sp. hordei isolate K1 genome re-assembly

ABSTRACT: Improvement of the Blumeria graminis f.sp. hordei isolate K1 genome assembly and annotation using Oxford Nanopore long-reads.

PROVIDER: PRJEB30373 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ERR3013816.fastq.gz	Fastqsanger.gz
	ERR3013817.fastq.gz	Fastqsanger.gz

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Gene expression analysis of RACB-transgenic barley plant lines in interaction with Blumeria graminis f.sp. hordei

Project description:To analyze RACB- and Bgh (Blumeria graminis f.sp. hordei)-dependently expressed genes we used wild type and transgenic barley misexpressing the susceptibility factor RACB

2017-03-27 | GSE69215 | GEO

Blumeria graminis f.sp. hordei DH14 proteome 2nd analysis incl. CSEPs

Project description:Large scale proteomics of Blumeria graminis f.sp. hordei DH14 has been conducted with a new ORF database containing candidate secreted effector proteins (CSEPs). With a comparative approach, CSEPs only deteted in Haustoria (the interacting cells) containing tissue were identified.

2012-12-06 | PRD000730 | Pride

LC-MSMS of Hordeum vulgare epidermis containing haustoria from Blumeria graminis f.sp. hordei

Project description:Blumeria graminis f.sp. hordei is an obligate biotrohic fungal pathogen causing powdery mildew in barley. As for other biotrophic fungi, haustorial structures are at the centre of the biotrophic interaction and molecular exchanges, delivering fungal effectors or virulence factors, and taking nutrient from the host. Haustoria are originiated by the fungus, following successful penetration of the initial penetration peg through the plant cell call. Haustorial structures mainly of fungal origin, but they are surrounding by a plant component, the extrauhaustorial membrane and matrix (EHM and EHMx) forming the extrahuastorial complex (EHMc). The plant protein make-up of the plant extrahaustorial components remained unexplored, and this is a first study trying to describe plant proteome associated with haustoria using samples enriched for these structures. Therefore, proteomes of haustoria enriched samples from the epidermis of barley leaves infected with Blumeria graminins f.sp. hordei, the causing agent of barley powdery mildew, were compared to infected epidermis and un-infected epidermis to identify haustoria associated plant proteins. Haustoria were enriched from infected epidermis by digesting epidermal cell walls with cell wall degrading enzymes prior to enrichment for haustorial structures. Proteins identified in these samples were compared to infected and uninfected epidermis samples using a non-targeted label free semi-quantitation method.

2019-11-01 | PXD012684 | Pride

Blumeria hordei K1 strain:K1

Project description:Blumeria graminis f. sp. hordei Genome sequencing Isolate K1

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Transcription profiling time series of leaves from Hordeum vulgare, Ingrid infected with Blumeria graminis f. sp. hordei or Blumeria graminis f. sp. tritici

Project description:Time course: Interaction of Blumeria graminis f. sp. hordei with Hordeum vulgare, Ingrid (leaf) and Blumeria graminis f. sp. tritici with Hordeum vulgare, Ingrid (leaf)

2009-06-10 | E-IPKG-7 | biostudies-arrayexpress

Transcription profiling time series of leaf epidermis from Hordeum vulgare Ingrid infected with Blumeria graminis f. sp. hordei and Blumeria graminis f. sp. tritici

Project description:Time course: Interaction of Blumeria graminis f. sp. hordei with Hordeum vulgare, Ingrid (leaf epidermis) and Blumeria graminis f. sp. tritici with Hordeum vulgare, Ingrid (leaf epidermis)

2009-06-10 | E-IPKG-8 | biostudies-arrayexpress

Iso-Seq analysis of barley CI 16151 and fast-neutron-derived, immune-compromised mutants infected with the powdery mildew fungus (Blumeria graminis f. sp. hordei; isolate 5874)

Project description:Purpose: The powdery mildew fungus, Blumeria graminis, is an obligate biotrophic pathogen of cereals and has significant impact on food security (Dean et al., 2012. Molecular Plant Pathology 13 (4): 414-430. DOI: 10.1111/j.1364-3703.2011.00783.x). Blumeria graminis f. sp. hordei (Bgh) is the causal agent of powdery mildew on barley (Hordeum vulgare L.). We sought to discover novel transcripts expressed following barley infection with blumeria.

2021-03-30 | GSE165730 | GEO

Transcription profiling of barley Mla1, Mla6, and Mla13 resistance specificities in response to inoculation with the Blumeria graminis f. sp. hordei (Bgh) isolates 5874 (AvrMla1, AvrMla6) and K1 (AvrMla1, AvrMla13)

Project description:A large-scale parallel expression analysis was conducted to elucidate Mla-specified responses to powdery mildew infection using 22K Barley1 GeneChip probe arrays. Our goal was to identify genes differentially expressed in incompatible (resistant) vs. compatible (susceptible) and Mla-specified Rar1-dependent vs. -independent interactions. A split-split-plot design with 108 experimental units (3 replications x 2 isolates x 3 genotypes x 6 time points) was used to profile near-isogenic lines containing the Mla1, Mla6, and Mla13 resistance specificities in response to inoculation with the Blumeria graminis f. sp. hordei (Bgh) isolates 5874 (AvrMla1, AvrMla6) and K1 (AvrMla1, AvrMla13).

2004-09-01 | E-MEXP-142 | biostudies-arrayexpress

Transcription profiling time series of leaves from Hordeum vulgare, Ingrid BC7 mlo5 infected with Blumeria graminis f. sp. hordei

Project description:Time course: Interaction of Blumeria graminis f. sp. hordei with Hordeum vulgare, Ingrid BC7 mlo5 (leaf)

2009-04-01 | E-IPKG-5 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type and pen3 mutant plants inoculated with powdery mildew organisms Erysiphe cichoracearum or Blumeria graminis hordei

Project description:Arabidopsis is a host to the fungal powdery mildew pathogen, Erysiphe cichoracearum, and a nonhost to Blumeria graminis f.sp. hordei, the powdery mildew pathogenic on barley. A screen for mutants that allowed increased entry by this inappropriate or nonhost pathogen on Arabidopsis led to the identification of PEN3. While pen3 mutants permitted both increased penetration and increased hyphal growth by B. g. hordei, they were unexpectedly resistant to E. cichoracearum. This resistance was correlated with the appearance of chlorotic patches and was salicylic acid-dependent. Consistent with this observation, microarray analysis revealed that the salicylic acid defense pathway was hyper-induced in pen3 relative to wild type following inoculation with either E. cichoracearum or B. g. hordei. The pen3 phenotypes result from a loss of function of AtPDR8, a ubiquitously and highly expressed ATP binding cassette transporter. PEN3 protein tagged with green fluorescent protein localized to the plasma membrane in uninfected cells. In infected leaves, the protein concentrated to high levels at infection sites and surrounded fungal penetration pegs. We hypothesize that PEN3 may be involved in exporting toxic substrates to sites of infection and that accumulation of these substrates intracellularly in the pen3 mutant may secondarily activate the salicylic acid pathway. Experiment Overall Design: Three week-old wild-type Col and mutant pen3 Arabidopsis thaliana plants were inoculated with Erysiphe cichoracearum, Blumeria graminis hordei, or not inoculated. 1 day post inoculation 16 rosettes were harvested per replicate. 4 replicates were perfomerd per treatment.

2007-10-04 | E-GEOD-3220 | biostudies-arrayexpress

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